We narrowed to 13,975 results for: CRISPR-Cas9

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pV1539

Plasmid

#111434

Purpose

Genedrive entry vector for Candida albicans

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

Plasmid

#211817

Purpose

AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

N-terminus of SpCas9-ABE8e and tracrRNA

Use

AAV

Expression

Mammalian

Promoter

chicken β-actin promoter and U6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265N2

Plasmid

#174001

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O2

Plasmid

#174002

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265L2

Plasmid

#174000

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

Plasmid

#211818

Purpose

AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

C-terminus of SpCas9-ABE8e and tracrRNA

Use

AAV

Expression

Mammalian

Promoter

chicken β-actin promoter and U6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLY54

Plasmid

#130923

Purpose

A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
pspFΔHTH::λN22plus
sfgfp

Use

Synthetic Biology

Tags

ASV tag and pspFΔHTH::λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLY9

Plasmid

#130907

Purpose

A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
pspFΔHTH::λN22plus
sfgfp

Use

Synthetic Biology

Tags

ASV tag and pspFΔHTH::λN22plus

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Anderson promoter: J23106, PpspA-2G6, and Ptet

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY59

Plasmid

#130927

Purpose

A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
sfgfp
pspFΔHTH::MCP

Use

Synthetic Biology

Tags

ASV tag and MCP (MS2 coat protein)

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Anderson promoter: J23106, PpspA-2G6, and Ptet

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLY57

Plasmid

#130926

Purpose

A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
pspFΔHTH::λN22plus
sfgfp

Use

Synthetic Biology

Tags

ASV tag and pspFΔHTH::λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLY56

Plasmid

#130925

Purpose

A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
pspFΔHTH::λN22plus
sfgfp

Use

Synthetic Biology

Tags

ASV tag and pspFΔHTH::λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLY55

Plasmid

#130924

Purpose

A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
pspFΔHTH::λN22plus
sfgfp

Use

Synthetic Biology

Tags

ASV tag and pspFΔHTH::λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLY53

Plasmid

#130911

Purpose

A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
pspFΔHTH::λN22plus
sfgfp

Use

Synthetic Biology

Tags

ASV tag and pspFΔHTH::λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLP857_α-syn:oScarlet KI donor

Plasmid

#239403

Purpose

AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

oScarlet

Use

AAV and CRISPR

Tags

oScarlet

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
RFN_EGFP_47

Plasmid

#60071

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
RFN_EGFP_84

Plasmid

#60076

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
RFN_EGFP_83

Plasmid

#60075

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
RFN_EGFP_82

Plasmid

#60074

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
RFN_EGFP_80

Plasmid

#60072

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only