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pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 mutUTR

Plasmid

#25039

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 mutant 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

substitution of 6nt within the miR-9 binding site…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

Plasmid

#187994

Purpose

Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

mucin2 D1D2D'D3 (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MLS-mir-17-92-Mut92

Plasmid

#64094

Purpose

expresses a mutated version of mir-17-92 (mutation of miR-92 seed)

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Mutation

mutation of miR-92 seed sequence

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
myc-hRAD18 d6BD

Plasmid

#68832

Purpose

Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173866

Purpose

Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-170)

Plasmid

#203426

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-208)

Plasmid

#203427

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-277)

Plasmid

#203428

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (170-439)

Plasmid

#203430

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-OSF-UFM1

Plasmid

#204565

Purpose

Expresses GST-Strep-FLAG-tagged UFM1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFM1 (UFM1 Human)

Expression

Bacterial

Mutation

delta84-85aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SPRR2A-pFastBac I

Plasmid

#182460

Purpose

Expression His-tagged SPRR2A in insect cells

Depositor

Lora Hooper

Article

Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

Insert

small proline rich protein 2A (SPRR2A Human)

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shBirc5

Plasmid

#160949

Purpose

Birc5 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Birc5 shRNA (Birc5 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-PKA-RIIβ-sREACh

Plasmid

#114113

Purpose

Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_EPS15_EH

Plasmid

#178475

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EPS15_EH (EPS15 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1CFP8

Plasmid

#41670

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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B*07:05:01

Plasmid

#203268

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*07:05:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-0-YFP

Plasmid

#41663

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP20

Plasmid

#41671

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 20 C-terminal-…

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+ oDam_f_GFPoNLS

Plasmid

#85819

Purpose

iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

oDam_f_GFPoNLS

Tags

oNLS (optimized nuclear localization signal) C te…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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