We narrowed to 18,078 results for: comp

1. pNOC_dCas9_sgRNA NC

   Plasmid

   #176259

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Promoter

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-ZNF768

   Plasmid

   #88524

   Purpose

   Donor Vector containing ZNF768 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF768 (ZNF768 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSICO-CPSF6-358-iRFP670

   Plasmid

   #110694

   Purpose

   Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

   Depositor

   Zandrea Ambrose

   Article

   Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

   Insert

   CPSF6-iRFP670 (CPSF6 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mutation

   Truncation of CPSF6 isoform at amino acid 358

   Promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-Coff/Fon-sRGECO

   Plasmid

   #137128

   Purpose

   Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVCT-Dnd1-EGFP

   Plasmid

   #106343

   Purpose

   Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1-EGFP (Dnd1 Mouse, Synthetic)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

   Plasmid

   #137165

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   3x-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-Con/Foff 2.0-BFP

   Plasmid

   #137130

   Purpose

   Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET16-mHP1alpha

   Plasmid

   #181910

   Purpose

   Bacterial protein expression vector for HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_DST-1/1

    Plasmid

    #91288

    Purpose

    Protein expression and purification of human SH3 domain construct DST-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DST-1/1 (DST Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-KIR3DX1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156774

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR3DX1 (KIR3DX1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mcry1K31R HA tag

    Plasmid

    #110297

    Purpose

    mammalian expression of full length mouse CRY1 mutant K31R

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mcry1 K31R (Cry1 Mouse)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    31 Lysines are changed to Argenine

    Promoter

    CMV

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 GIL-11 myc TEV TwinStrep

    Plasmid

    #199627

    Purpose

    Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

    Depositor

    Jurgen Scheller

    Article

    Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

    Insert

    GIL-11
    

    Use

    Tags

    TwinStrep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. phBG-P53DD

    Plasmid

    #149707

    Purpose

    In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

    Depositor

    Erika Sasaki

    Article

    Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    deleted amino acid 14-301

    Promoter

    T7

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-SIRPD-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156563

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIRPD (SIRPD Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. INCTbiosyn-p35S-GFP(rc)13

    Plasmid

    #127526

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-EGFP-C1-HsNot1iso1_1093-1317_W

    Plasmid

    #147892

    Purpose

    Mammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot1iso1_1093-1317 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-CTLA4-FLN-ELP-His-ybbr

    Plasmid

    #168038

    Purpose

    Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
    

    Use

    Tags

    3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MBP-hnRNPA2_deltaLC

    Plasmid

    #98664

    Purpose

    Expresses MBP-tagged hnRNPA2 without the LC domain

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    G190Stop

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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