We narrowed to 13,664 results for: sequence
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Plasmid#186067PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only
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CFP(159-238)-gamma-2 in pcDNAI/Amp
Plasmid#55777PurposeA C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.DepositorInsertCFP(159-238)-gamma-2 (GNG2 Aequorea victoria, Human)
TagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTR-379 tNGFR-P2A-CD48
Plasmid#186073PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-701pUC19-tNGFR-P2A-FOXP3
Plasmid#186114PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC9C1
Plasmid#132247PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC9C1 (SLC9C1 Human)
ExpressionMammalianAvailable SinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
CL20_mEGFP_CREBBP_ZNF500
Plasmid#205797PurposeExpress mEGFP-tagged fusion protein, CREBBP_ZNF500 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
Tags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-GFP
Plasmid#66083PurposeG protein alpha-s internally tagged with EGFP and EE epitopeDepositorInsertG-alpha-s-EE-EGFP (Gnas Rat)
TagsEGFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
ExpressionMammalianAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30D
Plasmid#214672PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice siteDepositorInsertMAPT (MAPT Human)
TagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30U
Plasmid#214673PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice siteDepositorInsertMAPT (MAPT Human)
TagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A51
Plasmid#132019PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A51 (SLC25A51 Human)
ExpressionMammalianAvailable SinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ai62(TITL-tdT) Flp-in replacement vector
Plasmid#61576PurposeRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locusDepositorInsertchromatin insulator flanked TRE-LSL-tdTomato
UseRecombinase-mediated cassette exchange using flp …Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
TagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-mXFPe-IFNGR2(30-337)
Plasmid#192787PurposeExpression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNGR2 (IFNGR2 Human)
TagsIg k-chain leader sequence and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_MFSD8
Plasmid#131882PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertMFSD8 (MFSD8 Human)
ExpressionMammalianAvailable SinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
CL20_mEGFP_FGFR3_TACC3
Plasmid#205821PurposeExpress mEGFP-tagged fusion protein, FGFR3_TACC3 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+(rInsp)-Ins-GLuc
Plasmid#89928PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
ExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoterrat Insulin II promoterAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX7_FOXO1
Plasmid#205891PurposeExpress mEGFP-tagged fusion protein, PAX7_FOXO1 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_TCF3_PBX1
Plasmid#205937PurposeExpress mEGFP-tagged fusion protein, TCF3_PBX1 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only