We narrowed to 25,854 results for: lars

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLARK_N

Plasmid

#146981

Purpose

Insect Expression of DmLARK

Depositor

Elisa Izaurralde

Insert

DmLARK (lark Fly)

Expression

Insect

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLarp_N

Plasmid

#146982

Purpose

Insect Expression of DmLarp

Depositor

Elisa Izaurralde

Insert

DmLarp (larp Fly)

Expression

Insect

Mutation

two non silent mutation N110T, P173L compared to …

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2

Plasmid

#116999

Purpose

Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Rivularia sp. PCC 7116 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC

Plasmid

#117000

Purpose

Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Rivularia sp. PCC 7116 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC

Plasmid

#116998

Purpose

Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Rivularia sp. PCC 7116 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Fibrilarin/pcDNA3

Plasmid

#67781

Purpose

Fibrilarin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor-Fibrilarin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Phamret-Fibrillarin/pcDNA3

Plasmid

#51954

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localization

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Tags

Fibrillarin

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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LRP4 (extracellular) scFv [N207/27]

Plasmid

#190537

Purpose

Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor WT

Plasmid

#12150

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Anti-ADAM22 (extracellular) [N57/2R]

Plasmid

#177564

Purpose

Mammalian Expression Plasmid of anti-ADAM22 (extracellular) (Mouse). Derived from hybridoma N57/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ADAM22 (extracellular) (Mus musculus) recombinant mouse monoclonal antibody (Adam22 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Lari-BFP2-TSERex

Plasmid

#123311

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Lari-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP-T2A-Flpo

Plasmid

#233212

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selection

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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LARG mCherry sspB in pcDNA3.1

Plasmid

#90459

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mCherry
sspB

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Mutation

intracellular domain *

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1 LARP7-FLAG 7SK

Plasmid

#200733

Purpose

Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK

Depositor

Claudio Alarcón

Insert

LARP7 and 7SK (LARP7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

U6

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
LARG mtq sspB in pcDNA3.1

Plasmid

#90460

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mTq
sspB

Expression

Mammalian

Mutation

R73Q and deletion a.a. (234-239)

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA8-Flag-LARS(1-976aa)

Plasmid

#139689

Purpose

Expresses N-teminal Flag tagged aa 1-976 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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FCK-CaMKII-Solaris-WPRE

Plasmid

#217480

Purpose

Negative response-polarity hybrid voltage indicator

Depositor

Peng Zou

Insert

Solaris

Use

Lentiviral

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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