-
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159671PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertExuL-NLS-hSpCas9-T2A-GFP
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔM)
Plasmid#80040PurposeExpression or G2A mutant of EPN-01*DepositorInsertEPN-01*(G2A)
UseTagsMycExpressionMammalianMutationGly2 mutated to AlaPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR[traF]+Opie2-PuroR[dsxM]
Plasmid#131618PurposeExpresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.DepositorInsertsPuroR[dsxM]
NeoR[traF]
UseTagsExpressionInsectMutationPromoterOpie2Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA[bTub].1026C
Plasmid#112691Purposeexpress gRNA targeting bTub under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub]
UseCRISPRTagsExpressionMutationPromoterdU6-3Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL2)
Plasmid#80050PurposeExpression of EPN-01 mutated in ALIX binding site.DepositorInsertEPN-01(deltaYP)
UseTagsMycExpressionMammalianMutationYP37AAPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔL1)
Plasmid#80042PurposeExpression of PTAP L domain mutant EPN-01*DepositorInsertpCMV-EPN-01*(ΔL2)
UseTagsMycExpressionMammalianMutationPTAP sequence in p6 mutated to AlaPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔL2)
Plasmid#80043PurposeExpression of YPxL L domain mutantd EPN-01*DepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationYP37 in p6 mutated to AAPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_B_1992_F
Plasmid#237358PurposeExpression plasmid for RSV B F from 1992DepositorInsertRSV B F from 1992 human codon optimized from Genbank Accesion OK649748
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_B_OR795252_F_N201S
Plasmid#237363PurposeExpression plasmid for RSV B F from OR795252DepositorInsertRSV B F human codon optimized from Genbank Accesion OR79525
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_B_1982_F
Plasmid#237357PurposeExpression plasmid for RSV B F from 1982DepositorInsertRSV B F from 1982 human codon optimized from Genbank Accesion MG642043
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_Long_G_31AACTdel
Plasmid#237350PurposeExpression plasmid for RSV Long GDepositorInsertRSV Long G from Genbank Accesion AY911262.1
UseTagsExpressionMammalianMutation31 amino acid cytoplasmic tail deletionPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_B_2019_F
Plasmid#237359PurposeExpression plasmid for RSV B F from 2019DepositorInsertRSV B F from 2019 human codon optimized from Genbank Accesion PP352433
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_B_MN365362_F_K68Q
Plasmid#237361PurposeExpression plasmid for RSV B F from MN365362DepositorInsertRSV B F human codon optimized from Genbank Accesion MN36536
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_B1_G_31AACTdel
Plasmid#237354PurposeExpression plasmid for RSV B1 GDepositorInsertRSV B1 G human codon optimized from Genbank Accesion AF013254
UseTagsExpressionMammalianMutation31 amino acid cytoplasmic tail deletionPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_B_OQ279862_F_N201T
Plasmid#237362PurposeExpression plasmid for RSV B F from OQ279862DepositorInsertRSV B F human codon optimized from Genbank Accesion OQ27986
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HDM_RSV_A2_G_31AACTdel
Plasmid#237352PurposeExpression plasmid for RSV A2 GDepositorInsertRSV A2 G human codon optimized from Genbank Accesion KT992094.1
UseTagsExpressionMammalianMutation31 amino acid cytoplasmic tail deletionPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY171_pPGK1_iRFP_WPRE
Plasmid#225351PurposeLentiviral vector for iRFP expressionDepositorInsertiRFP670
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
aTY173_pPGK1_mTagBFP2_WPRE
Plasmid#225352PurposeLentiviral vector for BFP expressionDepositorInsertBFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His_TEV_MsbA
Plasmid#191476PurposeExpresses MsbA with a TEV protease cleavable N terminal His TagDepositorInsertMsbA
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL CusS NarX Chimera
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322_ZA-ampR-PSEAB-2A
Plasmid#135230PurposeAAC6 experimental evolutionDepositorInsertaac6 Pseudomonas sp. ABAC61
UseTagsExpressionBacterialMutationPromoterpZAAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJPT008-ZF6 M-synTF-1x FLAG
Plasmid#202498PurposeExpress "M" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAG and Myristoylation siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT007-ZF6 synTF-1x FLAG
Plasmid#202497PurposeExpress "soluble" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT006-1x FLAG-HaloTag-PPF
Plasmid#202496PurposeExpress "PPF" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Palmitoylation-Palmitoylation-Farnesy…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT010-ZF6 1x FLAG-synTF-G
Plasmid#202500PurposeExpress "G" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAG and Geranylgeranyl siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT004-1x FLAG-HaloTag-G
Plasmid#202495PurposeExpress "G" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Geranylgeranyl siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-NgPglO
Plasmid#198204PurposepSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tagDepositorInsertNgPglO
UseTagsFlag tagExpressionBacterialMutationPromoterpBADAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
1137B=Hsp70Bb-Cas9
Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*-(ΔLF)
Plasmid#80045PurposeExpression of Vpr binding mutant EPN-01*DepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationVpr binding site LF45 in p6 mutatedPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL1+L2)
Plasmid#80051PurposeExpression of EPN-01 mutated in both L domainsDepositorInsertEPN-01(deltaPTAP/deltaYP)
UseTagsMycExpressionMammalianMutationPTAP10AAAA, YP37AAPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔI)
Plasmid#80041PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationchanged Leu33 in I3-01 to AlaPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only