We narrowed to 682 results for: bacillus
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Plasmid#87375PurposeAn ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assemblyDepositorInsertSpyTag
UseSynthetic BiologyTagsHis6 tagAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG-B-mSpyCatcher-C
Plasmid#87374PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assemblyDepositorInsertmSpyCatcher
UseSynthetic BiologyAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBsaHFm
Plasmid#85103PurposeHeme-FMN domain of P450 BM3 variant (A74G)DepositorInsertHeme-FMN domain of P450 BM3
UseCloning vectorMutationA74GAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL0_115 [pBC1 ORI]
Plasmid#199015PurposeLevel 0 partDepositorInsertpBC1 ORI
UseSynthetic BiologyAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBsaFa
Plasmid#85104PurposeFAD domain of P450 BM3 variant (C773S/C810S)DepositorInsertFADdomain of P450 BM3
UseCloning vectorMutationC773S/C810SAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pP3-GFP
Plasmid#53436PurposeControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeDepositorInsertP3 promoter
UseSynthetic BiologyTagsnoneExpressionBacterialMutationnonePromoterP3Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterPhyper spankAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyPromoterPftsH and Synthetic promoter PRhl0L0L12Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRBBm59
Plasmid#48115PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
ExpressionBacterialPromoterPxylAAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRBBm69
Plasmid#48116PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - sacB
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMZ1001
Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyPromoterPftsH and Synthetic promoter PRpa0002Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCG-D-mSpyCatcher-His6-E
Plasmid#87376PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assDepositorInsertmSpyCatcher
UseSynthetic BiologyTagsHis6 tagAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-479.wt
Plasmid#13527DepositorInsertB. subtilis DEAD-box helicase YxiN
Tagsintein-chitin binding domainExpressionBacterialMutationnoneAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRBBm76
Plasmid#48117PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
AGG1829 OpIE2-Bn
Plasmid#242438PurposeInsect OpIE2 promoter expressing barnaseDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK014 [Marker_SacB_PartType4]
Plasmid#229251PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SacB_PartType4DepositorInsertSacB
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK015 [Marker_SacB_PartType5-7]
Plasmid#229252PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SacB_PartType5-7DepositorInsertSacB
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK016 [Marker_SacB_PartType8-10]
Plasmid#229253PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SacB_PartType8-10DepositorInsertSacB
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-BASU-His
Plasmid#218451PurposeE coli expression vector for Halo-BASU-His biotin ligaseDepositorInsertHaloTag-BASU-His fusion protein
ExpressionBacterialPromoterT7Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB
Plasmid#225064PurposeExpression of HalM-modified HalA2 in human cellsDepositorInsertHalA2
TagsMultiple tags due to polycistronic expressionExpressionMammalianMutationWTPromoterCMVAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNAPf-BASU-His
Plasmid#218450PurposeE coli expression vector for SNAPf-BASU-His biotin ligaseDepositorInsertSNAPf-BASU-His fusion protein
ExpressionBacterialPromoterT7Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TM1442-pET-21a
Plasmid#12650DepositorAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_13
Plasmid#60584Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_13
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp
Plasmid#159617PurposeExpression plasmid for Sfp phosphopantetheinyl transferaseDepositorInsertSfp
Tags6HISExpressionBacterialAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
ExpressionBacterialMutationA309GAvailable SinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
TagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAPB02
Plasmid#65628PurposeA transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015
Plasmid#202025PurposeExpresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coliDepositorInsertBacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
ExpressionBacterialMutationGvpB S9G-R31L-R85LAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS
Plasmid#196484PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
ExpressionMammalianPromoterCMV (synthetase), Pol III tRNA promoter (tRNA)Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterPhyper spankAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pThuS
Plasmid#171674PurposeThuS expression in E. coliDepositorInsertGlycosyltransferase ThuS
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJT42_GalL_BE-PAPAPA
Plasmid#145035PurposeExpresses BE-PAPAPA in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only