Skip to main content
Addgene

We narrowed to 8,902 results for: BLI;

Showing: 251 - 300 of 8902 results
  1. pKAM-ME-Flag-BFP

    Plasmid
    #101868
    Purpose
    Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers
    Depositor
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pFRT-DestFLAGHA_RNAseH1

    Plasmid
    #65783
    Purpose
    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells
    Depositor
    Insert
    RNaseH1 (RNASEH1 Zebrafish, Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pEXV3-Myc-H-RAS_N17

    Plasmid
    #39527
    Depositor
    Insert
    HRAS N17 (HRAS Human)
    Use
    Eukaryotic
    Tags
    Myc
    Expression
    Mutation
    N17, missing aa1-4 (MTEY)
    Promoter
    Available since
    Sept. 26, 2012
    Availability
    Academic Institutions and Nonprofits only
  4. CMYA5tnC/pDEST26-N-FLAG

    Plasmid
    #98130
    Purpose
    Express CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. TTN/pAS2-1

    Plasmid
    #98127
    Purpose
    titin in DNA-binding domain (BD) vector, used for yeast-two hybrid
    Depositor
    Insert
    TTN (TTN Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pcDNA3.1-mouse cdk6 R31C/D163N

    Plasmid
    #75172
    Purpose
    Mammalian expression of mouse cdk6 with R31C and D163N
    Depositor
    Insert
    cyclin dependent kinase 6 (Cdk6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R31C, D163N
    Promoter
    CMV
    Available since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pGfa2-EGFP

    Plasmid
    #53128
    Purpose
    Expression of EGFP in mouse astrocytes
    Depositor
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    EGFP and mP1
    Expression
    Mammalian
    Mutation
    contains -2163 to +47 (the human gfa2 segment), w…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. pGfa2-luc3

    Plasmid
    #53129
    Purpose
    luciferase reporter for human gfa2 promoter
    Depositor
    Insert
    gfa2 promoter fragment (GFAP Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains -2163 to +47 (the human gfa2 segment), w…
    Promoter
    gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. CMYA5tnT/pACT2

    Plasmid
    #98126
    Purpose
    CMYA5 (aa 3859 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybrid
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    rs10043986 (NP_705838.3:p.Pro4063Leu)
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. FGFR2 V564F 3xFlag

    Plasmid
    #110108
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Valine 564 to Phenylalanine (V564F)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. FGFR2 K659M 3xFlag

    Plasmid
    #110112
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Lysine 659 to Methiionine (K659M)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. FGFR2 E565A 3xFlag

    Plasmid
    #110109
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Glutamic acid 565 to Alanine (E565A)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. FGFR2 K641R 3xFlag

    Plasmid
    #110111
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Lysine 641 to Arginine (K641R)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. FGFR2 N549H 3xFlag

    Plasmid
    #110106
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Asparagine 549 to Histidine (N549H)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. FGFR2 N549K 3xFlag

    Plasmid
    #110107
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Asparagine 549 to Lysine (N549K)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. FGFR2 L617V 3xFlag

    Plasmid
    #110110
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Leucine 617 to Valine (L617V)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. CMYA5tnT/pDEST26-N-FLAG

    Plasmid
    #98131
    Purpose
    Express CMYA5 (aa 3859 – 4069) Leu allele (4063) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    rs10043986 (NM_153610.4:c.12188C>T)
    Promoter
    CMV
    Available since
    Aug. 3, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  18. pDownstream1Lox

    Plasmid
    #164574
    Purpose
    Integration of 1 LoxN site in the genome of P. berghei
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pUpstream2Lox

    Plasmid
    #164573
    Purpose
    Integration of 2 LoxN sites in the genome of P. berghei
    Depositor
    Inserts
    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Synthetic, Human)
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid
    #83107
    Purpose
    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide
    Depositor
    Inserts
    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    Use
    Lentiviral
    Tags
    2A self cleaving peptide
    Expression
    Mammalian
    Mutation
    R24C and p64 isoform
    Promoter
    hPGK and hPGK (single transcript)
    Available since
    July 3, 2017
    Availability
    Academic Institutions and Nonprofits only
  21. pSPIKE-F

    Plasmid
    #101174
    Purpose
    chimeric spike resembling fungal ITS region to be used in metagenomics
    Depositor
    Insert
    chimeric fungal ITS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
  22. pSPIKE-P

    Plasmid
    #101172
    Purpose
    chimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomics
    Depositor
    Insert
    V4 16S rRNA chimeric spike
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
  23. pSPIKE-E

    Plasmid
    #101173
    Purpose
    chimeric spike resembling a fragment of eukaryotic 18S rRNA to be used in metagenomics
    Depositor
    Insert
    chimeric 18S rRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
  24. pCS2+mScarlet3-C Cloning Vector

    Plasmid
    #220616
    Purpose
    To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    mScarlet3 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. pCMV-AAVS1-TALEN-L

    Plasmid
    #159299
    Purpose
    TALEN (left) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Insert
    TALEN targeting the AAVS1
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  26. pCMV-AAVS1-TALEN-R

    Plasmid
    #159300
    Purpose
    TALEN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Insert
    TALEN targeting the AAVS1
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  27. pZFN-AAVS1_ELD

    Plasmid
    #159297
    Purpose
    ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Insert
    ZFN targeting the AAVS1 locus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  28. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
  29. pZFN-AAVS1-KKR

    Plasmid
    #159298
    Purpose
    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Insert
    ZFN targeting the AAVS1 locus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  30. Flag-APEX3-C Cloning Vector

    Plasmid
    #220615
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Flag-APEX3 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  31. Rab5-L38R-mCherry

    Plasmid
    #102785
    Purpose
    Point mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308D
    Depositor
    Insert
    Rab5a (RAB5A Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Leucine 38 changed to Arginine
    Promoter
    CMV
    Available since
    Jan. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  32. CROPseq-3P5P

    Plasmid
    #219680
    Purpose
    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 251 - 300 of 8902 results