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We narrowed to 2,968 results for: PPL

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Showing: 251 - 300 of 2968 results

  2. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid
    #177722
    Purpose
    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFluc
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177722-plasmid-map-sequence-id-352603

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  12. pPLN-hFluc-DM-eGFP-KDEL

    Plasmid
    #177728
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Tags
    KDEL, Prolactin Signal Sequence, and eGFP
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177728-plasmid-map-sequence-id-352602

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  21. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid
    #177723
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177723-plasmid-map-sequence-id-352618

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  23. pplss-mRFP-KDEL-pcDNA3.1/Hygro

    Plasmid
    #242808
    Purpose
    ER marker
    Depositor
    Christoph Kaether
    Insert
    mRFP with ER targeting signal sequence and C-terminal KDEL-ER retention signal
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    242808-plasmid-map-sequence-id-488738

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  29. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid
    #188385
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188385-plasmid-map-sequence-id-371050

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  38. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Article
    Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Expression
    Mammalian
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

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  40. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

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  41. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  42. pMBPcyto-TEV-BseRI

    Plasmid
    #157739
    Purpose
    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    His10 + MBP (cytoplasmic expression; no signal se…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157739-plasmid-map-sequence-id-334003

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  46. GS2.1/EC

    Plasmid
    #132568
    Purpose
    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).
    Depositor
    Mathias Pribil
    Article
    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111
    Insert
    egg cell promoter, Cas9-mApple, pds3 sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    A. thaliana egg cell promoter
    Available Since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    132568-plasmid-map-sequence-id-262634

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Showing: 251 - 300 of 2968 results