Skip to main content
Addgene
Showing: 251 - 300 of 6475 results

  17. pMAT_M6_ITYAAV

    Plasmid
    #197086
    Purpose
    This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197086-plasmid-map-sequence-id-392959

    Enlarge

  20. pAAV_Cbh_DIO_hTyrNull_HATag

    Plasmid
    #205284
    Purpose
    Truncated version of human Tyrosinase as control virus for Tyrosinase expression
    Depositor
    Matthias Prigge
    Insert
    hTyrosinase (truncated)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205284-plasmid-map-sequence-id-415327

    Enlarge

  21. pAAV_Ef1a_DIO_hTyrNull_HATag

    Plasmid
    #205285
    Purpose
    Truncated version of human Tyrosinase as control virus for Tyrosinase expression
    Depositor
    Matthias Prigge
    Insert
    hTyrosinase (truncated)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205285-plasmid-map-sequence-id-412579

    Enlarge

  22. HT152_pAAV_EF1a_fDiO_pAce

    Plasmid
    #208610
    Purpose
    Expressing pAce in a Flp-dependent manner
    Depositor
    Adam Cohen
    Insert
    pAce
    Use
    AAV
    Tags
    mNeon Green
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208610-plasmid-map-sequence-id-414378

    Enlarge

  27. pENN.AAV.SV40-si-CS

    Plasmid
    #187861
    Purpose
    a human siRNA-expressing plasmid targeting CS
    Depositor
    Yan Zeng
    Insert
    si-CS (CS Human)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    187861-plasmid-map-sequence-id-370178

    Enlarge

  30. pAAV.CMV.PI-si-CS

    Plasmid
    #187857
    Purpose
    a human siRNA-expressing plasmid targeting CS
    Depositor
    Yan Zeng
    Insert
    si-CS (CS Human)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    187857-plasmid-map-sequence-id-370021

    Enlarge

  31. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid
    #183078
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    V600E (T>A), S607S (TCC>AGT)
    Promoter
    None for primary insert. PGK for puromycin resist…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183078-plasmid-map-sequence-id-363420

    Enlarge

  37. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid
    #98219
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    human synapsin
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98219-plasmid-map-sequence-id-256052

    Enlarge

  41. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

    Enlarge

  42. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid
    #197029
    Purpose
    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized ecDHFR-enOsCas12f1-ecDHFR
    Use
    AAV
    Tags
    Expression
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197029-plasmid-map-sequence-id-388970

    Enlarge

  43. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113194-plasmid-map-sequence-id-216314

    Enlarge

  44. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

    Enlarge

  45. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid
    #105540
    Purpose
    AAV expression of EGFP-Cre from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP-Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105540-plasmid-map-sequence-id-202434

    Enlarge

  46. pAAV-ATLASsnCre

    Plasmid
    #232351
    Purpose
    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.
    Depositor
    Don Arnold
    Insert
    ATLASsnCre
    Use
    AAV
    Tags
    ALFA-Tag
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232351-plasmid-map-sequence-id-471577

    Enlarge

Showing: 251 - 300 of 6475 results