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pSB6

Plasmid

#82028

Purpose

Hybrid McpC[1-350]-YSYEH-Tar[272-553]

Depositor

Victor Sourjik

Publication

Bi et al ACS Synth Biol. 2016 Jun 10.

Insert

Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-YSYEH-Tar[272-553]

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDestattB_ubi:EGFP (pCM272)

Plasmid

#68339

Purpose

Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

Depositor

Christian Mosimann, Leonard Zon

Publication

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

EGFP

Use

Zebrafish transgenesis

Promoter

ubi

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Rtn2b-RHD272-472-GFP

Plasmid

#186606

Purpose

Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

Met-Gly-Ser-Lys peptide added at N-terminus for c…

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-RHD272-472-mNeon

Plasmid

#186605

Purpose

Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

Met-Gly-Ser-Lys peptide added at N-terminus for c…

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMCS.71/2272

Plasmid

#22732

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox71-MCS-Lox2272

Use

Cre/Lox

Mutation

This is a basal exchange vector for RMCE containi…

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pMCS.71/2272.Hygro

Plasmid

#22731

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox71-FRT-PGK-hygro-FRT-Lox2272

Use

Cre/Lox

Mutation

This is a basal exchange vector for RMCE. The vec…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pBT272_(pRosa26-GTET)

Plasmid

#36882

Depositor

Liqun Luo

Publication

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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3410 pBM272 Bcl-XL

Plasmid

#8772

Depositor

Stanley Korsmeyer

Publication

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Expression

Yeast

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-PGK2

Plasmid

#23625

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PGK2 (PGK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pENTR1A-Huwe1

Plasmid

#37431

Depositor

Jean Cook

Insert

Huwe1 (HUWE1 Human)

Use

Gateway Cloning

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-Casp7 C186A-Flag

Plasmid

#11816

Depositor

Guy Salvesen

Publication

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 7 catalytic mutant (CASP7 Human)

Tags

Flag

Expression

Mammalian

Mutation

C186A

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Flag-HA-BAP1

Plasmid

#22539

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pT2/Onc

Plasmid

#21360

Depositor

David Largaespada

Publication

Collier et al Nature. 2005 Jul 14. 436(7048):272-6.

Insert

Sleeping Beauty Transposon with splice acceptors and murine stem cell virus LTR

Use

Transposon

Available since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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Apple-53BP1trunc

Plasmid

#69531

Purpose

Fluorescent reporter for DNA damage

Depositor

Ralph Weissleder

Publication

Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

Apple fluorescent protein

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA3 HA Sam68 deltaKH

Plasmid

#17688

Depositor

David Shalloway

Publication

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

aa 157-256 deleted (KH domain)

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAAV-U6-sgRNA-CMV-GFP

Plasmid

#85451

Purpose

Express sgRNA in mammalian cells

Depositor

Hetian Lei

Publication

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pCMV-EGFP

Use

AAV

Promoter

pCMV-EGFP

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Publication

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP

Expression

Mammalian

Available since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

18 Articles

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pET23b-Casp3 C163A-His

Plasmid

#11822

Depositor

Guy Salvesen

Publication

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C163A

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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