Addgene

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pGAN213

Plasmid

#129270

Purpose

Control for measuring background levels of yTRAP reporter protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

min pCYC1

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGAN101

Plasmid

#131123

Purpose

Plasmid for overexpression of prion domains to induce prions

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Tags

mKate2

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRY1001

Plasmid

#21787

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

CFP-Ste12 (STE12 A. victoria, Budding Yeast)

Use

Tags

CFP

Expression

Yeast

Mutation

contains 1000 bp UTR (Ste12 promoter) upstream of…

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1V5-His Snk/Plk2 T239A

Plasmid

#22219

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Use

Tags

Expression

Mammalian

Mutation

single amino acid substitution mutant, threonine …

Promoter

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1V5-His Snk/Plk2 T239D

Plasmid

#22220

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Use

Tags

Expression

Mammalian

Mutation

single amino acid substitution mutant, threonine …

Promoter

Available since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Use

Tags

6xHA

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1V5-His Snk/Plk2 D223N

Plasmid

#22218

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Use

Tags

Expression

Mammalian

Mutation

single amino acid substitution mutant, aspartic a…

Promoter

Available since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSK260

Plasmid

#129252

Purpose

dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35 NM yTRAP sensor reporting on mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

pSUP35, min pCYC1

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK226

Plasmid

#129268

Purpose

dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Rnq1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Rat SERCA3 (pMT2)

Plasmid

#75185

Purpose

Mammalian expression of ATP2A3

Depositor

Gary Shull, Jonathan Lytton

Article

Burk et al J Biol Chem. 1989 Nov 5;264(31):18561-8.

Insert

ATP2A3 (Atp2a3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

adenovirus major late promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 A. victoria, Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Promoter

Available since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-Paxillin

Plasmid

#176106

Purpose

Focal Adhesion marker

Depositor

Dorus Gadella

Article

Arts et al Front Immunol. 2021 May 18;12:667213. do

Insert

Paxillin (PXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pC5Kan-P2A

Plasmid

#51814

Purpose

P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message

Depositor

Barry Ganetzky

Article

Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:

Insert

P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)

Use

Shuttle vector for insect expression vectors

Tags

Expression

Mutation

codons altered for Drosophila and to eliminate re…

Promoter

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD_His B_QuasAr2

Plasmid

#64134

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mTurquoise2-Paxillin

Plasmid

#176107

Purpose

Focal Adhesion marker

Depositor

Dorus Gadella

Article

Arts et al Front Immunol. 2021 May 18;12:667213. do

Insert

Paxillin (PXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAN201

Plasmid

#129204

Purpose

yTRAP sensor of [RNQ+], detects aggregation of Rnq1

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

RNQ1 (RNQ1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD_His B_QuasAr1

Plasmid

#64135

Purpose

Fluorescent reporter for membrane voltage with improved brightness

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge