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Addgene

We narrowed to 643 results for: Adh1

Showing: 101 - 120 of 643 results
  1. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. GFP-X3

    Plasmid
    #115568
    Purpose
    Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif
    Depositor
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Three repeats of the Gcn5 consensus motif are tag…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. GFP-X2

    Plasmid
    #115567
    Purpose
    GFP tethered to two repeats of the Gcn5 consensus motif
    Depositor
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. GFP-X1

    Plasmid
    #115566
    Purpose
    GFP tethered to a single repeat of the Gcn5 consensus motif
    Depositor
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Single Gcn5 consensus motif repeat tethered to C-…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Insert
    RGR-GFP
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. GFP-X2DM

    Plasmid
    #115571
    Purpose
    GFP-X2 where both of the consensus motif's lysines are mutated to arginine
    Depositor
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. GFP-X2R2

    Plasmid
    #115570
    Purpose
    GFP-X2 where the second consensus motif's lysine is mutated to arginine
    Depositor
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. GFP-X2R1

    Plasmid
    #115569
    Purpose
    GFP-X2 where the first consensus motif's lysine is mutated to arginine
    Depositor
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pG2J

    Plasmid
    #102882
    Purpose
    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
    Depositor
    Inserts
    OsTIR1
    mGFP-AID
    Use
    Tags
    AID(71-114)
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 and TEF1
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pMZ379

    Plasmid
    #74215
    Purpose
    Combined sgRNA/Cas9 vector with NatMX marker
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pMZ377

    Plasmid
    #74214
    Purpose
    Combined sgRNA/Cas9 vector with LEU2 marker
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 643 results