We narrowed to 11,071 results for: Kars;

1. pKAR2-Br512g3.1

   Plasmid

   #171200

   Purpose

   Expresses Br512g3.1 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al medRxiv 2022.08.09.22278605

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (vHP47…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sapphireCKAR

   Plasmid

   #118468

   Purpose

   cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ER-AKAR4

   Plasmid

   #64733

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   AKAR4
   

   Tags

   Cerulean, N-terminal 27 amino acids from CYP450, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EKARet-cyto

   Plasmid

   #96895

   Purpose

   FLIM sensor for ERK activation in cytosol

   Depositor

   Ryohei Yasuda

   Article

   Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

   Insert

   FLIM Sensor for ERK Activity in Cytosol
   

   Tags

   EGFP and sREAChet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_SynP_DIO_tAKARα _WPRE

   Plasmid

   #196675

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   tAKARα
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-CKAR2

   Plasmid

   #123348

   Purpose

   Improved FRET-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   CKAR2
   

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-PRKAR1B

   Plasmid

   #23376

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PRKAR1B (PRKAR1B Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. Cyto-ABKAR

   Plasmid

   #61510

   Purpose

   Expression of AMPK biosensor

   Depositor

   Jin Zhang, Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   AMPK Biosensor
   

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mito-ABKAR

   Plasmid

   #61509

   Purpose

   Expression of AMPK biosensor targeted to the mitochondria

   Depositor

   Jin Zhang, Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   AMPK Biosensor
   

   Tags

   Mitochondrial targeting signal (DAKAP1-30)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+)-blueAKAR

    Plasmid

    #118470

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-sapphireCKAR(T/A)

    Plasmid

    #118469

    Purpose

    Negative-control mutant for sapphireCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_SynP_DIO_tAKARα- T391A_WPRE

    Plasmid

    #196676

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    tAKARα-T391A
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223-PRKAR2A

    Plasmid

    #23789

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PRKAR2A (PRKAR2A Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pKAR2-Br512g3.2

    Plasmid

    #171201

    Purpose

    Expresses Br512g3.2 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al medRxiv 2022.08.09.22278605

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (vHP47…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-blueCKAR

    Plasmid

    #118472

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-AKAR2-CR

    Plasmid

    #40255

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    AKAR2
    

    Tags

    His6 and Xpress

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-JNKAR1

    Plasmid

    #61625

    Purpose

    FRET-based biosensor for monitoring JNK activity

    Depositor

    Jin Zhang

    Article

    Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    JNKAR1
    

    Tags

    Citrine and ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-ABKAR

    Plasmid

    #140051

    Purpose

    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-GR-AKAR3

    Plasmid

    #173015

    Purpose

    Improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-AKAR3
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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