We narrowed to 9,122 results for: Pol

1. pOpen-BstpolLF

   Plasmid

   #165553

   Purpose

   Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Bst DNA Polymerase, Large Fragment
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pOpen-Bstpol

   Plasmid

   #165543

   Purpose

   Full length DNA polymerase from Bacillus stearothermophilus.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Bst DNA Polymerase, Full Length
   

   Use

   Synthetic Biology

   Available Since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pOpen-TthpolA

   Plasmid

   #165533

   Purpose

   DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Tth DNA polymerase
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pOpen-9N7polA (CT)

   Plasmid

   #165502

   Purpose

   9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   9 degrees N-7 DNA Polymerase chain terminating
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. AE-Polyubiquitin-GCaMP6s

   Plasmid

   #106868

   Purpose

   Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

   Depositor

   Omar Akbari

   Article

   Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

   Insert

   AE-Polyubiquitin-GCAaMP6s
   

   Expression

   Insect

   Promoter

   Ae. aegypti polyubiquitin (PUb)

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. T. aq RNA Polymerase

   Plasmid

   #215349

   Purpose

   Express T. aq RNA Polymerase subunits in E. coli cells

   Depositor

   Julius Lucks

   Article

   Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

   Insert

   RNA polymerase
   

   Tags

   6x His

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPolII-Luc

   Plasmid

   #17223

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   PolII promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pLKO.1P POLE1_1

   Plasmid

   #160762

   Purpose

   Suppress POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLE1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-ECOpolA

   Plasmid

   #165532

   Purpose

   DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   E. coli DNA Polymerase I
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTol2CG NBT:NRGIIIa:polyA

    Plasmid

    #193012

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa (NRG1 Human)
    

    Use

    Transposon transgenesis

    Expression

    Mammalian

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P POLE1_2

    Plasmid

    #160764

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FRB-polySH33

    Plasmid

    #175233

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-Pol-II 15

    Plasmid

    #35177

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains the C-terminal repeats 16-52

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pLKO.1P POLE2

    Plasmid

    #160766

    Purpose

    Suppress POLE2

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97309

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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