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We narrowed to 4,942 results for: codon optimized

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Showing: 101 - 120 of 4942 results

  3. PET.SUMO eIF4G1 1-200

    Plasmid
    #158791
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.
    Depositor
    Benjamin Blencowe
    Publication
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (codon optimized)
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158791-plasmid-map-sequence-id-309813

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  4. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid
    #185910
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185910-plasmid-map-sequence-id-363739

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  5. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185911-plasmid-map-sequence-id-367364

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  7. pSm-Nuclease

    Plasmid
    #132431
    Purpose
    Expresses Serratia marcescens nuclease in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    Serratia marcescens nuclease (codon optimized)
    Tags
    None
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132431-plasmid-map-sequence-id-258770

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  11. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72249-plasmid-map-sequence-id-131869

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  12. pYPQ158

    Plasmid
    #69307
    Purpose
    Gateway entry vector with hSpCas9 (Human codon optimized)
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9 (Human codon optimized)
    Use
    CRISPR
    Tags
    3XFLAG
    Mutation
    NLS fusion
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69307-plasmid-map-sequence-id-122145

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  16. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid
    #163991
    Purpose
    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging
    Depositor
    Hao Wu
    Publication
    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLRP1 CARD (NLRP1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    163991-plasmid-map-sequence-id-324596

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  17. pYPQ151

    Plasmid
    #69302
    Purpose
    Gateway entry vector with pcoCas9D10A
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pcoCas9D10A (Plant codon-optimized)
    Use
    CRISPR
    Tags
    2XFLAG
    Mutation
    D10A mutation; NLS fusion
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69302-plasmid-map-sequence-id-122140

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  18. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  19. pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

    Plasmid
    #199567
    Purpose
    Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer
    Depositor
    Andrea Cimarelli, Yuxin Song
    Publication
    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Trim69 isoform B (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Mutation
    codon optimized
    Available since
    May 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199567-plasmid-map-sequence-id-392223

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  20. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid
    #184563
    Purpose
    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
    Depositor
    Kacper Rogala
    Publication
    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:
    Insert
    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    All lysines were mutated to arginines. Codon-opti…
    Promoter
    CMV
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184563-plasmid-map-sequence-id-376975

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Showing: 101 - 120 of 4942 results