We narrowed to 106 results for: gfp donor template

1. pAP3041

   Plasmid

   #137083

   Purpose

   Plasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFP

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

   Insert

   GFP11::3xFlag::tagRFP
   

   Use

   Pcr template for donor dna

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

   Plasmid

   #170185

   Purpose

   Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Inserts

   Ec86 RT
   Retron Ec86 msr-msd
   BFP-to-GFP conversion donor template An
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and hU6

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

   Plasmid

   #170184

   Purpose

   Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Inserts

   Sa163 RT
   Retron Sa163 msr-msd
   BFP-to-GFP conversion donor template An
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and hU6

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH3

   Plasmid

   #52528

   Purpose

   serves as PCR-template to generate HR donors for C-terminal GFP-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   eGFP and Blasticigin resistance cassette
   

   Use

   Blunt cloning kit

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-trGluc

   Plasmid

   #158959

   Purpose

   DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   trGluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-PtH2B

   Plasmid

   #133390

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   No promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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