We narrowed to 4,673 results for: pcr
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Plasmid#66295PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGLP1R (GLP1R Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OCT4-eGFP-PGK-Puro
Plasmid#31937PurposeOct4 targeting donor vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOCT4 (POU5F1 Human)
UseTargeting donorTagseGFPAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_NE1m
Plasmid#123308PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based NE sensor GRAB_NE1m
UseAAVMutationcontains a glycine-to-threonine mutation at posit…PromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRD2-Tango
Plasmid#66269PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRD2 (DRD2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-28a_6H-MMLV_RT_D524N-6H
Plasmid#166945PurposeBacterial expression of M-MLV reverse transcriptaseDepositorInsertgag-pol
Tags6X HisExpressionBacterialMutationD524N, H8YPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Oct4-IRES-eGFP-PGK-Neo
Plasmid#48681PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOct4 (Pou5f1 Mouse)
UseCRISPRTagsGFPAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_DA1m
Plasmid#113049Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neuronsDepositorHas serviceAAV9InsertGPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based NE sensor GRAB_NE1h
UseAAVPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB-gDA3m
Plasmid#208698PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neuronsDepositorHas serviceAAV1InsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
UseAAVPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P07
Plasmid#124788PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UsePcr templateTagseGFPPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR1316
Plasmid#111126PurposeExpresses FANCA.DepositorAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCrlM
Plasmid#122444PurposeExpresses myc tagged mouse reelin ORF in mammalian cellsDepositorAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCrl
Plasmid#122443PurposeExpresses entire mouse reelin ORF in mammalian cellsDepositorAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPRi_Mxi1_yl_NHEJ
Plasmid#91249PurposeCRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HRDepositorInsertsCodon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMaCTag-P34
Plasmid#120030PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_VPR_yl
Plasmid#107677PurposeCRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-VPR
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only