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pJK_proB_eGFP

Plasmid

#59845

Purpose

Constitutively expresses eGFP with proB promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proB

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pTD103luxI_sfGFP -R

Plasmid

#48887

Purpose

Positive feedback plasmid from oscillator without luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

sfGFP and luxI

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCSegfpcherry

Plasmid

#22465

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP-mCherry

Use

Injection construct

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PPP1CB_ALK

Plasmid

#205899

Purpose

Express mEGFP-tagged fusion protein, PPP1CB_ALK from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PPP1CB_ALK (PPP1CB, ALK Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#174666

Purpose

Doxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a-3xFLAG (Arid3a Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p3xEGFP-N1

Plasmid

#122163

Purpose

Expression of 3xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

3xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.ARID3a.IRES.GFP

Plasmid

#169300

Purpose

Constitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ZC3H4_POLR2I

Plasmid

#205956

Purpose

Express mEGFP-tagged fusion protein, ZC3H4_POLR2I from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ZC3H4_POLR2I (ZC3H4, POLR2I Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFPH7

Plasmid

#83554

Purpose

Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

ADH2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH2

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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ss-cfSGFP2

Plasmid

#37535

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

ss-cfSGFP2 (cysteine-free SGFP2)

Use

Tags

signal sequence of α1-antitrypsin (M1-K34)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Use

Tags

Expression

Bacterial

Mutation

Y39Stop

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CLTC_VMP1

Plasmid

#205793

Purpose

Express mEGFP-tagged fusion protein, CLTC_VMP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CLTC_VMP1 (CLTC, VMP1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CSF2RA_CRLF2

Plasmid

#205798

Purpose

Express mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CSF2RA_CRLF2 (CSF2RA, CRLF2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-8x

Plasmid

#172284

Purpose

Expressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-8x

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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p6xEGFP-N1

Plasmid

#122166

Purpose

Expression of 6xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

6xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET:GACT:GFP

Plasmid

#60734

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK1_eGFP

Plasmid

#59853

Purpose

Constitutively expresses eGFP with proK1 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK1

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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oxGFP

Plasmid

#68069

Purpose

C-terminal fusion to oxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Use

Tags

oxGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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GwKif2CGFP

Plasmid

#21479

Purpose

Expression of KifC2-GFP in mammalian cells.

Depositor

Don Arnold

Article

Chu et al J Biol Chem. 2006 Jan 6. 281(1):365-73.

Insert

Kinesin family member C2 (Kifc2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

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