We narrowed to 9,242 results for: ef1

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pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

Plasmid

#134857

Purpose

Expresses EGFP-tagged synaptic vesicle marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 3C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Article

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-UBC-irfp luc-EF1 Hygro

Plasmid

#129438

Purpose

express irfp-luc chimera

Depositor

Oskar Laur

Insert

irfp-luc

Use

Lentiviral

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G

Plasmid

#204190

Purpose

Doxycycline inducible lentiviral vector for human TFAP2C overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

TFAP2C (TFAP2C Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

Plasmid

#207123

Purpose

Lentiviral vector to express human METTL3

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

hMETTL3 (METTL3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

Plasmid

#185451

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

Plasmid

#194231

Purpose

Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

EF1a-LCB1-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX2-1-178-EGFP-Hygro

Plasmid

#238094

Purpose

Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX2 (DTX2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-178 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

Plasmid

#139236

Purpose

mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PX552-mScn2a-3xV5-EF1-smFP-flag

Plasmid

#182561

Purpose

For mouse Nav1.2 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

Plasmid

#203847

Purpose

Flp-dependent expression of Chronos fused to EGFP

Depositor

Ivo Spiegel

Article

Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

Has Service

AAV2

Insert

Chronos

Use

AAV

Tags

EGFP

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

Plasmid

#226011

Purpose

CasRx guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-ReaChR-P2A-dTomato

Plasmid

#114368

Purpose

expresses a Cre-dependent red light-shifted channelrhodopsin variant

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

ReaChR-P2A-dTomato

Use

AAV

Tags

dTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a-FRT-MCS-WPRE-SV40 - GCH15

Plasmid

#230822

Purpose

AAV cloning vector for Flpo-dependent expression under the EF1a promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV; Cloning vector

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

Plasmid

#114369

Purpose

expresses Flpo recombinase-dependent GtACR2

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP (NEWENTRY )

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

Plasmid

#114370

Purpose

expresses Flpo recombinase-dependent GtACR1

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR1-EYFP (NEWENTRY )

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

Plasmid

#122966

Purpose

Expresses 3XFlag-tagged human CEP164 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

CEP164 (CEP164 Human)

Use

Lentiviral

Tags

3XFlag

Promoter

EF1 alpha

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-GCaMP6M

Plasmid

#137120

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-UBC-dendra 2LUC-EF1 Hygro

Plasmid

#129439

Purpose

express dendra2-luc chimera

Depositor

Oskar Laur

Insert

dendra2-luc

Use

Lentiviral

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

Plasmid

#125148

Purpose

Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Insert

AcrIIA4-P2A-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Jaws-KGC-GFP-ER2

Plasmid

#153537

Purpose

AAV-mediated expression of Jaws-GFP-ER2 under the EF1α promoter (1.1kb short version).

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

EF1α1.1

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPB EF1a CCR6-eLOV-TEVcs-Gal4

Plasmid

#173119

Purpose

Expresses CCR6 eLOV receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-Gal4

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-UBC-DSRED-LUC-EF1 Hygro

Plasmid

#129437

Purpose

express dsred-luc chimera

Depositor

Oskar Laur

Insert

dsred-luc

Use

Lentiviral

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

Plasmid

#197066

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

CRISPR

Tags

FKBP and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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