We narrowed to 12,118 results for: pcDNA

1. pcDNA3-GFPLaminA-L530P

   Plasmid

   #32710

   Depositor

   Wendy Bickmore

   Article

   Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

   Insert

   Lamin A (LMNA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L530P

   Promoter

   CMV

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5/FRT/TO_STf_LamB1

   Plasmid

   #181999

   Purpose

   CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   STf-LamB1
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-LTK-V5/HIS

   Plasmid

   #200116

   Purpose

   Expression of human LTK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human LTK receptor tyrosine kinase (LTK Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG-Luc-N

   Plasmid

   #58790

   Purpose

   Express LucN alone in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   firefly Luciferase 1-398
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T576C,T591C to destroy MfeI and EcoRI sites

   Promoter

   CMV

   Available Since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-Luc-C

   Plasmid

   #58791

   Purpose

   Express LucC alone in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   firefly Luciferase 394-550
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-HA-mSTING (V154M)

   Plasmid

   #214159

   Purpose

   Transient expression of gain-of-function STING mutant

   Depositor

   Nan Yan

   Article

   Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

   Insert

   STING (V154M) (Sting1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N153S

   Promoter

   CMV

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PDI-QUAD-V5-pcDNA3.1

   Plasmid

   #153550

   Purpose

   PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.

   Depositor

   Julie Atkin

   Article

   Parakh et al iScience. 2020 May 22;23(5):101097. doi:

   Insert

   Protein disulphide isomerase
   

   Tags

   V5 tag

   Mutation

   C53S, C56S, C397S, C400S

   Promoter

   pCMV

   Available Since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+_FH-AGO2-WT

   Plasmid

   #92006

   Purpose

   Expresses FLAG-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-YFP-PKD2

   Plasmid

   #31821

   Depositor

   Alexandra Newton

   Article

   Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

   Insert

   YFP-Protein Kinase D2 (PRKD2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3/hArf3(WT)-mCherry

    Plasmid

    #79420

    Purpose

    Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf3 (ARF3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-RNMT

    Plasmid

    #112705

    Purpose

    Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-HA-mRFP-pcDNA3.1-

    Plasmid

    #52510

    Purpose

    expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    mRFP
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    engineered variant of tetrameric red fluorescent …

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-Flag-KIAA0947

    Plasmid

    #49248

    Purpose

    Mammalian expression of flag-tagged human KIAA0947

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    KIAA0947 (ICE1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 PM-Ras-LOCKR-S

    Plasmid

    #216498

    Purpose

    FRET-based reporter for Ras activity, plasma membrane localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    PM-Ras-LOCKR-S
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 proHB-EGF CS

    Plasmid

    #11601

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    Constitutively secreted HB-EGF: C-terminal amino …

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3/mArf1(Q71L)-HA

    Plasmid

    #79410

    Purpose

    Expresses C-terminally HA-tagged Arf1(Q71L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf1 (Arf1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 71 to Leucine

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HtrA2-EGFP

    Plasmid

    #14121

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA HA FAK Y397F

    Plasmid

    #50503

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y397F

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-HDAC4.3SA-FLAG

    Plasmid

    #30486

    Depositor

    Tso-Pang Yao

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S246/467/632A

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-PalmPalm-ClopHensor

    Plasmid

    #25940

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    PalmPalm-ClopHensor
    

    Tags

    Gap20 and Strep-II

    Expression

    Mammalian

    Mutation

    N-term (20AA) of Gap-43

    Available Since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA4.TO-ORF73-2xCSTREP

    Plasmid

    #136232

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF73
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-FLAG-VSVG

    Plasmid

    #80606

    Purpose

    Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    VSV-G
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA5/FRT/TO V5 DNAJB6b

    Plasmid

    #19528

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6b (DNAJB6 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA4.TO-ORF50-2xCSTREP

    Plasmid

    #136211

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF50
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-TYRO3-V5/HIS

    Plasmid

    #200118

    Purpose

    Expression of human TYRO3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human TYRO3 receptor tyrosine kinase (TYRO3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA Set9 mutant

    Plasmid

    #24085

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H297A

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-SypHluorin 2x (S2x)

    Plasmid

    #37004

    Depositor

    Stephen Heinemann, Yongling Zhu

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin 2x (Syp Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Two copies of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4/HisMaxB-YAP1

    Plasmid

    #18978

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-mouse cdk6 wt

    Plasmid

    #75170

    Purpose

    Mammalian expression of mouse cdk6

    Depositor

    Martine Roussel

    Insert

    cyclin dependent kinase 6 (Cdk6 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA5/FRT/TO DNAJB1

    Plasmid

    #19468

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA5 TEX264wt-cGFP

    Plasmid

    #220211

    Purpose

    Mammalian expression of wild type TEX264 fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-HA-p53 2KR

    Plasmid

    #78799

    Purpose

    To overexpress p53 2KR in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    p53 (TP53 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K291R, K292R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PREX2 pcdna3.1 V5/his

    Plasmid

    #41555

    Depositor

    Ramon Parsons

    Article

    Fine et al Science. 2009 Sep 4;325(5945):1261-5.

    Insert

    PREX2a (PREX2 Human)
    

    Tags

    V5 and his

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.1-MS2-HA-HsRck_AC

    Plasmid

    #148417

    Purpose

    Mammalian Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HsRck (DDX6 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-Lyn-PlcR

    Plasmid

    #181830

    Purpose

    Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR
    

    Tags

    ECFP, EYFP, and N-terminal targeting sequence fro…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Kv7.4 (1-695)/pcDNA3.1

    Plasmid

    #111453

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    8xHis and EGFP

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-695)

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-GFP-p48

    Plasmid

    #11616

    Depositor

    Steven Johnson

    Insert

    Interferon-Stimulated Gene Factor 3 (IRF9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only