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Plasmid#136335PurposeMammalian expression of human p115-RhoGEF with N-terminal V5DepositorInsertARHGEF1 (ARHGEF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-wtSrc
Plasmid#140294PurposeExpresses HA-tagged wild-type human Src in mammalian cells for structure-function studiesDepositorInsertSrc wildtype (SRC Human)
UseTags2X HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SAR1-V5-His6
Plasmid#67451PurposeMammalian expression of hSar1 with C-term V5 and His6 tagDepositorInsertSAR1 (SAR1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-2C-NLS in pCSDest
Plasmid#126638PurposeExpresses LbCas12a-2C-NLS in mammalian cellDepositorInsertLbCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP_Hu_p115-RhoGEF(WT)
Plasmid#136337PurposeMammalian expression of human p115-RhoGEF with N-terminal EGFPDepositorInsertARHGEF1 (ARHGEF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 cDNA wt pDEST40
Plasmid#42646Purposedestination vector for expression of human TLR4 in mammalian cellsDepositorInsertTLR4 (TLR4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorInsertSAR1 (SAR1A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2-GAL9
Plasmid#149520PurposeExpress APEX2-Galectin-9 in mammalian cellsDepositorInsertLGALS9 (LGALS9 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsV5 Epitope TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST/N1-hBIN1-GFP
Plasmid#27305DepositorInsertbridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/v5-DEST hGlut2
Plasmid#18086DepositorInsertGlut2 (SLC2A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Flag-FBW7K404R
Plasmid#200717PurposeThe plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
UseTagsFlagExpressionMammalianMutationFBW7 K404 is mutated to Alanine.PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-N (LEU2)
Plasmid#177798PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-KD
Plasmid#140297PurposeExpresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationcontains only the SH4 and KD regionsPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APEX2-nDEST
Plasmid#214992PurposeGateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tagDepositorTypeEmpty backboneUseLentiviralTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only