We narrowed to 4,276 results for: GCA

1. KIF2A D8.2 gRNA

   Plasmid

   #90721

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KIF2A

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   KIF2A (Guide Designation D8.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. WEE1 F9.4 gRNA

   Plasmid

   #90940

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human WEE1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   WEE1 (Guide Designation F9.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPR v2-sgAMPK alpha1 clone2

   Plasmid

   #162120

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA0055

   Plasmid

   #131774

   Purpose

   Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer CASS5a guide (gCASS5a) and 5' sgRNA
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSF1 KO

   Plasmid

   #200207

   Purpose

   gRNA for HSF1 KO

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   HSF1 KO (Hsf1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ATP1A1_G8_Dual_sgRNA

   Plasmid

   #178104

   Purpose

   Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ANAPC1 A2.3 gRNA

   Plasmid

   #90529

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ANAPC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ANAPC1 (Guide Designation A2.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJG488

   Plasmid

   #91196

   Purpose

   WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nTaCas9_H840A+gUbi8+gUbi1+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCascade-IS2

   Plasmid

   #140625

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15416-BEAR-mScarlet-target-BFP

    Plasmid

    #162996

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-mScarlet plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

    Plasmid

    #138985

    Purpose

    IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

    Plasmid

    #138984

    Purpose

    IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-puro-mU6-CNPshRNA1

    Plasmid

    #126611

    Purpose

    Expresses shRNA against human CNP under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    CNP ShRNA-1 (CNP Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid

    #173214

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR sgHAL

    Plasmid

    #102315

    Purpose

    genetic depletion of HAL

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgHAL (HAL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-shEsr1

    Plasmid

    #120720

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Satta et al Int J Neuropsychopharmacol. 2018 Apr 1;2

    Insert

    Esr1 shRNA (Esr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse U6

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. WPXL LEFTY2 gRNA

    Plasmid

    #101924

    Purpose

    LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    LEFTY2 enhancer targeting gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET a sfMatryoshCaMP6s

    Plasmid

    #100020

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PPP2R5D B12.5 gRNA

    Plasmid

    #90854

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PPP2R5D

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PPP2R5D (Guide Designation B12.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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