We narrowed to 22,403 results for: his
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Plasmid#167269PurposeOverexpression of SNAP-tag in E. coliDepositorInsertSNAP-tag
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC
Plasmid#13005DepositorInserthuman sulfatase 1 delta CC (SULF1 Human)
ExpressionMammalianMutationchanged Cysteine 87 to AlanineAvailable SinceOct. 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE1
Plasmid#73018PurposeExpresses BE1 with N-terminal His6 tag in E. ColiDepositorInsertBE1
TagsHis6 tagExpressionBacterialPromoterT7Available SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
TagsHis6 TagExpressionBacterialPromoterT7Available SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBT-Mb(GDx-Clo_L10)
Plasmid#183406PurposeExpresses Monobody GDx-Clo_L10 in E. coliDepositorInsertHis tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
TagsHis tag-Avi tag-TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc397
Plasmid#161955PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHHDepositorInsertpSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
ExpressionBacterialPromoterPBADAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUG-331
Plasmid#104756PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC35_MMZ1_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-332
Plasmid#104757PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC35_MMZ2_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-333
Plasmid#104758PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC35_MMZ3_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-334
Plasmid#104759PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC35_MMZ4_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-335
Plasmid#104760PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC36_MMZ1_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-336
Plasmid#104761PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC36_MMZ2_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-337
Plasmid#104762PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC36_MMZ3_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-338
Plasmid#104763PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC36_MMZ4_EL4
TagsHIS-Ubq, HA-E2ExpressionBacterialPromoterT7Available SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500
Plasmid#92230PurposeExpression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue V86 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a SpyCatcher-SnoopCatcher
Plasmid#72324PurposeBacterial expression of SpyCatcher linked to SnoopCatcher.DepositorInsertpET28a SpyCatcher-SnoopCatcher
TagsN-terminal His6 tagExpressionBacterialMutation35 amino acids at the N-term of SpyCatcher have b…Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGAm (FlincG3)
Plasmid#49202PurposeFluorescent reporter for cGMPDepositorInsertFlincG3
TagsHis tag + other tagsExpressionBacterial, Insect, and Mamm…MutationMet to Lys in cpEGFP region similar to CGaMP3PromoterCMVAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only