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pLEX301-ARF6-T157N-TagRFP

Plasmid

#162030

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

T151N

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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FB013

Plasmid

#119710

Purpose

TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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LV-hGFAP-GFP-T2A-SOX2

Plasmid

#183908

Purpose

Expression of GFP and human SOX2 under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

Insert

SOX2 (GFAP, SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN_4A

Plasmid

#227435

Purpose

Expression of the R-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN 4A (Pten Rat)

Use

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CAG_G-PTEN

Plasmid

#227436

Purpose

Expresses the G-PTEN sensor under a CAG promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor, mEGFP-PTEN-sREACh (Pten Rat)

Use

Tags

mEGFP, and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN

Plasmid

#227433

Purpose

Expresses the R-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN (Pten Rat)

Use

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN_4A

Plasmid

#227434

Purpose

Expresses the G-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN 4A (Pten Rat)

Use

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

4A mutation.

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304 ARF6-R-WT mNG

Plasmid

#233255

Purpose

Expression of mNeonGreen-tagged ARF6 WT

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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hARF6-R WT TurboID V5 T2A BFP

Plasmid

#233256

Purpose

Expression of BFP-tagged ARF6 WT for Turbo ID experiments

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-30nmEML-mRFP

Plasmid

#231182

Purpose

Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vector

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB Ubiqutin-BFP

Plasmid

#202426

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

Ubiquitin, TagBFP

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Tags

Expression

Mutation

T161A

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1

Plasmid

#123326

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2

Plasmid

#123327

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 G40N-P2A-Blast

Plasmid

#123321

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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ACA8-mYFP

Plasmid

#87245

Purpose

fluorescent labelling of ACA8

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

ACA8 (ACA8 Mustard Weed)

Use

Tags

YFP

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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ACA8-YFPc

Plasmid

#87243

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

ACA8 (ACA8 Mustard Weed)

Use

Tags

YFPc

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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ACA8-YFPn

Plasmid

#87241

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

ACA8 (ACA8 Mustard Weed)

Use

Tags

YFPn

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pTH731-2µ-RLuc/minCFLuc

Plasmid

#40601

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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