We narrowed to 69,826 results for: nin

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pACYC_HA-ZmSAE1_ZmSAE2-FLAG

Plasmid

#91944

Purpose

Bacterial expression of maize SAE1 and SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pOGG009

Plasmid

#113983

Purpose

Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Gentamicin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23-SCP1-ccdB

Plasmid

#131628

Purpose

Modified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)

Depositor

Kevin White

Article

Liu et al Genome Biol. 2017 Nov 20;18(1):219. doi:

Type

Empty backbone

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SCP1

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-EmGFP-15b

Plasmid

#138280

Purpose

Express tTALE-EmGFP

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-EmGFP

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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stathmin-CFP

Plasmid

#86783

Purpose

Mammalian expression of stathmin-CFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG

Plasmid

#188984

Purpose

Express chimadanin with TAG at 28 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 position

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pC0.008

Plasmid

#119618

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

eYFP

Use

Synthetic Biology

Mutation

bp 242 T to A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1)

Plasmid

#80049

Purpose

Expression of EPN-01 mutated in TSG101 binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP)

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-oPRE-L2

Plasmid

#32415

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

oPRE

Use

Gateway cloning vector

Mutation

WPRE with deletion of X protein promoter and muta…

Available Since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode1

Plasmid

#83678

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode1

Use

Retroviral

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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LoriT

Plasmid

#163691

Purpose

Modified L4440 containing now CamR and oriT for conjugation

Depositor

Baris Tursun

Article

Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

Insert

oriT

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFH45

Plasmid

#125810

Purpose

Level0 nSpCas9-ABE7.10 base changer, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nSpCas9-ABE7.10 base changer, human codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD-Cyc1t (17-21)

Plasmid

#101700

Purpose

Cyc1t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTEF2 (01-11)

Plasmid

#101685

Purpose

pTEF2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 10 mut B-Myb

Plasmid

#25966

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (Mybl2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonines 267, 408, 497, 519, 522 and 52…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
GWF148

Plasmid

#133617

Purpose

GNCR1-BFP-CAAX

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

GNCR1-BFP-CAAX

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mClover3-EC18157

Plasmid

#137076

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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GGE038

Plasmid

#120785

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsDown_Zeta-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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