We narrowed to 5,868 results for: nls

1. p1xNLS-FRB-mCherry-hsp86-BSD

   Plasmid

   #85795

   Purpose

   Expression of 1xNLS mislocaliser in P. falciparum

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   1xNLS Mislocaliser
   

   Use

   P. falciparum expression

   Tags

   FRB*, mCherry

   Expression

   Mutation

   Promoter

   Pfhsp86

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

   Plasmid

   #134333

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Efa
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-Synapsin-miRFP670-3XNLS-WPRE

   Plasmid

   #159952

   Purpose

   nucleus marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   miRFP670-3XNLS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGFAP-NLS-HA-Dre-P2A-BFP

   Plasmid

   #51277

   Purpose

   Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-Dre-P2A-BFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   hGFAP

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

   Plasmid

   #225245

   Purpose

   Mammalian expression of iPASnFR-H in the nucleus

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-MED1trunc-IDR-YFP-NLS

   Plasmid

   #145275

   Purpose

   Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MED1-IDR truncated (MED1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIG-V5-Snail2-IRES-nls-GFP

   Plasmid

   #44282

   Depositor

   Martin Cheung

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

   Insert

   V5-Snail2 (SNAI2 Chicken)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE-enhancer and chicken beta-actin promoter

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. P2A-NLS-tdtomato-EPG backbone

   Plasmid

   #215548

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

   Plasmid

   #128833

   Purpose

   Knock-in of nuclear EYFP into mouse Elf5 locus

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Nuclear EYFP into Elf5 locus (Elf5 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG413 GAL ORF1 Shuffle1 NLS

    Plasmid

    #145794

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Payero et al Journal of the South Carolina Academy of

    Insert

    ORF1 Shuffle1 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Enhanced Nucleur Localization Signal

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA15-Ubc-NLS- scFV-tdTomato

    Plasmid

    #199446

    Purpose

    Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    scFV-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

    Plasmid

    #80004

    Purpose

    miRFP709 GAF domain fused to FKBP and NLS

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    FKBP
    

    Use

    Tags

    GAF domain of miRFP709 and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xnls-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129626

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. FokI(inactive)-mCherry-LacI-NLS

    Plasmid

    #215100

    Purpose

    Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    FokI(inactive)-mCherry-LacI-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FokI(inactive) mutant D69A.

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-NLS-Beta-catenin-S33Y

    Plasmid

    #138537

    Purpose

    For making Beta-catenin-S33Y RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Beta-catenin
    

    Use

    In vitro transcription

    Tags

    2XFLAG and 2xNLS

    Expression

    Mutation

    changed serine 33 to tyrosine

    Promoter

    T7

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. EYFP-NLS-actin-K68/284R

    Plasmid

    #60616

    Purpose

    Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

    Depositor

    Primal de Lanerolle

    Article

    Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    K68R, 284R

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCSD_N-SpCas9-1-573-FRB-1xNLS

    Plasmid

    #107298

    Purpose

    Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 1-573) fused to FRB
    

    Use

    CRISPR

    Tags

    FRB and SV40 NLS

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 1-573)

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

    Plasmid

    #136513

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    codon-optimized AcrIIA5
    

    Use

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

    Plasmid

    #138374

    Purpose

    Booster-PKA for the generation of mice

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    lox-mCherry-lox-Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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