2,226 results

1. CMV-IgK-pHluorin-TM-mRuby

   Plasmid

   #185546

   Purpose

   Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   IgK-pHluorin-TM-mRuby
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. rSirt3 shRNA (pLKO.1 vector)

   Plasmid

   #216807

   Purpose

   shRNA against rat Sirt3

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

   Insert

   Sirt3 (Sirt3 Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSLC17A6/vGlutT2/pCMV6-entry

   Plasmid

   #196939

   Purpose

   Plasmid expressing an antigen targeting the VGluT2 transporter

   Depositor

   James Trimmer

   Insert

   vGlutT2/ SLC17A6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET SUMO-syt1 (96-421)

   Plasmid

   #213619

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 (96-421)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 (96-421) (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET SUMO-syt1 (96-421) I367C

   Plasmid

   #213620

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 (96-421) I367C (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   C277A; I367C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET SUMO-syt1 5W

   Plasmid

   #213623

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 5W

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 5W (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   M173W, F231W, F234W, C277A, V304W, Y364W, I367C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET SUMO-syt1 s7+

   Plasmid

   #213624

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 s7+

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 s7+ (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET SUMO-FL-syt1 5W

   Plasmid

   #213629

   Purpose

   Bacterial expression of SUMO-tagged rat FL-syt1 5W

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt1 5W (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   M173W, F231W, F234W, C277A, V304W, Y364W, I367C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET SUMO-Doc2β C2AB (G361C)

   Plasmid

   #213631

   Purpose

   Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   Doc2β C2AB (G361C) (Doc2b Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   G361C, all original cysteines mutated to alanines

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB-Neo-rTOM20-TagBFP

    Plasmid

    #210332

    Purpose

    Overexpression of rTOM20

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    rTOM20 (Tomm20 Rat)
    

    Use

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_Caspase-11_rat_catalytic domain

    Plasmid

    #214329

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (Casp4 Rat)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a_Pro-IL-18_rat

    Plasmid

    #214333

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (Il18 Rat)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFBMeCP2G.10 (pc1577)

    Plasmid

    #211728

    Purpose

    Expresses MeCP2 (aa1-76) GFP tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MeCP2 aa1-76
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promotor

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFB-MeCP2.9Ch (pc2385)

    Plasmid

    #211720

    Purpose

    Expresses MeCP2 (aa163-309) mCherry tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MeCP2 aa163-309
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFB-MeCP2G.9 (pc1574)

    Plasmid

    #211719

    Purpose

    Expresses MeCP2 (aa163-309) GFP tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MeCP2 aa163-309
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFB-MeCP2G.8 (pc1573)

    Plasmid

    #211718

    Purpose

    Expresses MeCP2 (aa310-492) GFP tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MeCP2 aa310-492
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFB-MeCP2Y.3 (pc1575)

    Plasmid

    #211717

    Purpose

    Expresses MeCP2 (aa1-163) YFP tagged in insect cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MeCP2 aa1-163
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMeCP2R.9 (pc2812)

    Plasmid

    #211574

    Purpose

    Expresses MeCP2 (aa163-309) GFP tagged in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

    Insert

    MeCP2 aa163-309
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CavB4/pFlag myc-CMV

    Plasmid

    #197302

    Purpose

    Plasmid expressing an antigen targeting the Cavbeta4 calcium channel

    Depositor

    Ricardo Dolmetsch

    Insert

    Cavb4
    

    Use

    Tags

    pFLAGmyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETSUMOSyt1C2AB(80-421)

    Plasmid

    #209005

    Purpose

    Recombinant protein expression of truncated syt1 construct

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 80-421 only

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-Dlx-SynaptoTAG

    Plasmid

    #210729

    Purpose

    AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.

    Depositor

    Wei Xu

    Article

    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

    Insert

    EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Rat, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Dlx

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKT-CNP-inact

    Plasmid

    #211804

    Purpose

    Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H73L H153L

    Promoter

    tet

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKT-CNP

    Plasmid

    #211803

    Purpose

    Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tet

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30b jGCaMP8f L27A

    Plasmid

    #204138

    Purpose

    Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    T7

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGP-CMV-jGCaMP8f L27A

    Plasmid

    #204140

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. dCas9-RPT6-FLAG

    Plasmid

    #205416

    Purpose

    Expresses dCas9-RPT6 with FLAG label

    Depositor

    Timothy Jarome

    Article

    Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

    Insert

    dCas9-RPT6 (Psmc5 Rat)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. An R57A, R59A CAAX pMT2

    Plasmid

    #206119

    Purpose

    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. cacna2d1 R241A pMT2

    Plasmid

    #206110

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R241A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. cacna2d1 R241A HA pMT2

    Plasmid

    #206111

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R241A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. An HA CAAX pMT2

    Plasmid

    #206116

    Purpose

    expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge