We narrowed to 9,389 results for: Coli

1. pSelect-7

   Plasmid

   #190244

   Purpose

   Encodes lacI- and pheA-targeting gRNAs for positive and negative selections of dCas9 activity.

   Depositor

   Marc Ostermeier

   Article

   Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

   Insert

   LacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSelect-9

   Plasmid

   #190245

   Purpose

   Encodes lacI- and aadA-targeting gRNAs for positive and negative selections of dCas9 activity.

   Depositor

   Marc Ostermeier

   Article

   Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

   Insert

   LacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKP2164

   Plasmid

   #167608

   Purpose

   Express ExbD V45A, L49A

   Depositor

   Kathleen Postle

   Article

   Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

   Insert

   exbD V45A, L49A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbD with V45A, L49A mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKP2171

   Plasmid

   #167609

   Purpose

   Express ExbD V45A, V47A

   Depositor

   Kathleen Postle

   Article

   Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

   Insert

   exbD V45A, V47A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbD with V45A, V47A mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKP2182

   Plasmid

   #167610

   Purpose

   Express ExbD V45A,P50A

   Depositor

   Kathleen Postle

   Article

   Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

   Insert

   exbD V45A, P50A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbD with V45A, P50A mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKP2190

   Plasmid

   #167612

   Purpose

   Express ExbD V47A, P50A

   Depositor

   Kathleen Postle

   Article

   Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

   Insert

   exbD V47A, P50A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbD with V47A, P50A mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKP2202

   Plasmid

   #167613

   Purpose

   Express ExbD V49A, P50A

   Depositor

   Kathleen Postle

   Article

   Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

   Insert

   exbD V49A, P50A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbD with V49A, LP50A mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKP2201

   Plasmid

   #167611

   Purpose

   Express ExbD V47A, L49A

   Depositor

   Kathleen Postle

   Article

   Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

   Insert

   exbD V47A, L49A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbD with V47A, L49A mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKB252

   Plasmid

   #170035

   Purpose

   Encodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   K6L9
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNK083b

    Plasmid

    #156366

    Purpose

    Expresses heterlogous ribosomal proteins

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    S16 ribosomal gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNK141o2

    Plasmid

    #156388

    Purpose

    Expresses orthogonal RBS mMaroon1 reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    mMaroon1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRB33

    Plasmid

    #165482

    Purpose

    pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    10xHis-FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKP920

    Plasmid

    #160814

    Purpose

    Expression of ExbB protein

    Depositor

    Kathleen Postle

    Article

    Ollis et al J Bacteriol. 2012 Jun;194(12):3069-77. d

    Insert

    exbB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNK141n2

    Plasmid

    #156387

    Purpose

    Expresses orthogonal RBS E2-Crimson reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNK141m2

    Plasmid

    #156386

    Purpose

    Expresses orthogonal RBS MiCy reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    MiCy
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNK141l2

    Plasmid

    #156385

    Purpose

    Expresses orthogonal RBS LSS-mKate2 reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    LSS-mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNK141j2

    Plasmid

    #156383

    Purpose

    Expresses orthogonal RBS Katushka-9-5 reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    Katushka-9-5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNK141i2

    Plasmid

    #156382

    Purpose

    Expresses orthogonal RBS mCherry reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNK141h2

    Plasmid

    #156381

    Purpose

    Expresses orthogonal RBS mScarlet-I reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNK141e2

    Plasmid

    #156378

    Purpose

    Expresses orthogonal RBS mEmerald reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    mEmerald
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNK141d2

    Plasmid

    #156377

    Purpose

    Expresses orthogonal RBS Sapphire reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    Sapphire
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNK141c2

    Plasmid

    #156376

    Purpose

    Expresses orthogonal RBS mCerulean reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNK141a2

    Plasmid

    #156374

    Purpose

    Expresses orthogonal RBS Sirius reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAB140j8

    Plasmid

    #156373

    Purpose

    Erythromycin-dependent sfGFP reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    ermCL-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNK108h

    Plasmid

    #156368

    Purpose

    Expresses heterlogous ribosomal proteins

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    S20, S16, S15 ribosomal genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNK083d

    Plasmid

    #156367

    Purpose

    Expresses heterlogous ribosomal proteins

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    S20, S16 ribosomal genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAB184c3

    Plasmid

    #156360

    Purpose

    Expresses heterlogous ribosomal proteins

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    S20, S16, S1, S15 ribosomal genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJOE4786.1

    Plasmid

    #135087

    Purpose

    Positive selection vector

    Depositor

    Josef Altenbuchner

    Article

    Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

    Insert

    LacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pClodAcytCh-PIP2PHGFP

    Plasmid

    #107867

    Purpose

    Bacterial expression of Cherry and GFP-PLCδ-PH domain

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    Cherry and PLCδ-PH domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pro D (dual)

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. relA+_NS1

    Plasmid

    #100916

    Purpose

    expresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genome

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

    Insert

    RelA fragment from E.coli
    

    Use

    Cyanobacteria cloning vector

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Ptrc::galP_NS1

    Plasmid

    #101843

    Purpose

    drives expression of galP transporter from NS1 locus

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    Ptrc promoter driving GalP transporter expression
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. cisRAJ31-cat

    Plasmid

    #69933

    Purpose

    Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    PllacO1-cisRAJ31-cat
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlLacO1

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPLS24

    Plasmid

    #17285

    Depositor

    Nancy Craig

    Article

    Sharpe et al EMBO J. 1998 Oct 1. 17(19):5822-31.

    Insert

    TnsD (tnsD E. coli transposon Tn7)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

34. pTK234

    Plasmid

    #13535

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-CTTY
    

    Use

    Tags

    See map

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKB254

    Plasmid

    #170037

    Purpose

    Encodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    H6L9
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Use

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228788

    Purpose

    EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAcBac3-EcTrp-TGA-EGFP**

    Plasmid

    #228786

    Purpose

    EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    EGFP**
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGL403

    Plasmid

    #119953

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge