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FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Incenp

Plasmid

#108480

Purpose

inducible expression of vsv-INCENP wt

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP7.v1 puro

Plasmid

#87794

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shDUSP19 puro

Plasmid

#87796

Purpose

Expresses an inducible short hairpin targeting human DUSP19 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shDUSP19 (DUSP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)

Plasmid

#70213

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

lacI

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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ZM (pyrFEKO-HYG TetR-T7RNAP)

Plasmid

#31455

Depositor

George Cross

Insert

Use

Floxed targeting vector for trypanosome

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pXTK071 [Con-Pro_tetA-v2-Broad_PartType8]

Plasmid

#229308

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_tetA-v2-Broad_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Insert

tetA-v2 Promoter (Broad)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-Tet1c-dCas9

Plasmid

#232178

Purpose

Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-Tet1v4-dCas9

Plasmid

#232180

Purpose

Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-TET1-S-MUT

Plasmid

#232943

Purpose

Expresses mutant form of the short isoform of TET1 in mammalian cells

Depositor

Jianjun Chen

Article

Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

Insert

TET1-S-MUT (TET1 Human)

Use

Lentiviral

Tags

Expression

Mutation

H1652Y and D1654A

Promoter

Available since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-AncHolozoan Sox

Plasmid

#231528

Purpose

Expresses reconstructed Ancestral Holozoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Holozoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-AncMetazoan Sox

Plasmid

#231527

Purpose

Expresses reconstructed Ancestral Metazoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Metazoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-I-Sox2HMG

Plasmid

#231533

Purpose

Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG flanked with N- and C-termini of Salhel-I

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-Sox-I

Plasmid

#231515

Purpose

Expresses Salpingoeca helianthica full-length Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Myflu-POU

Plasmid

#231523

Purpose

Expresses Mylnosiga fluctuans full-length POU in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-hCic

Plasmid

#231529

Purpose

Expresses chimeric human Cic in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Human Cic HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Sox2-HMG

Plasmid

#231532

Purpose

Expresses mouse Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-II-Sox2HMG

Plasmid

#231534

Purpose

Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG flanked with N- and C-termini of Salhel-II

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Salhel-I

Plasmid

#231513

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-POU

Plasmid

#231522

Purpose

Expresses Salpingoeca helianthica full-length POU in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge