We narrowed to 63,651 results for: cloning
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Plasmid#105804PurposeConcomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.DepositorTypeEmpty backboneUseFlp-in competentTagsVN173 (I152L); VC155ExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmEGFP-1
Plasmid#36409DepositorTypeEmpty backboneUseFluorescent protein cassette for sub-cloningTagsmEGFPPromoterno promoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_LacZ
Plasmid#25893PurposeGateway entry vector containing LacZ.DepositorInsertLacZ
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-15:ACTB-mEGFP
Plasmid#87425PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACTB Homology Arms with mEGFP-linker (ACTB Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDONR223 SARS-CoV-2 S
Plasmid#149329PurposeGateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pET15bpATnp
Plasmid#121137PurposeThis vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.DepositorInsertProtein A-Tnp
TagshisExpressionBacterialPromoterT7Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-HA-USP39
Plasmid#22581DepositorPublicationAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-HA-UCHL3
Plasmid#22564DepositorPublicationAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd MafA-I-nGFP
Plasmid#19412DepositorInsertv-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)
UseAdenoviral and Gateway CloningTagsGFP and IRESAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA 3.1(-) mouse C/EBP delta
Plasmid#12559DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
ExpressionMammalianMutationS2G introduced during cloningAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWPI-IRES-Puro-Ak-ACE2-TMPRSS2
Plasmid#154987PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDonor_sU6
Plasmid#69351PurposesgRNA scaffold and synthetic U6 promoterDepositorInsertsU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRPromotersynthetic U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-dTom-nlsdTom
Plasmid#135630PurposeAAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertdTom-nlsdTom
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5
Plasmid#83896PurposeGi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInserthM4Di
UseAAVExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfuNca-MCS
Plasmid#190820PurposeMultiple cloning site between Nitzschia captiva promoter and termination regionsDepositorInsertsMultiple cloning site with SpeI and PstI
Bleomycin resistance gene
ExpressionBacterialAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_DA1m
Plasmid#113049Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neuronsDepositorHas serviceAAV9InsertGPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pForge-KI-MMEJ-Cas9-DualGuide
Plasmid#258792PurposeMMEJ Dual NucleaseDepositorInserthU6/7SK PITCh dual-guide cloning sites
UseCRISPRPromoterhU6Available sinceAug. 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
OgeuIscB ωRNA-v2
Plasmid#220958Purposehuman U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloingDepositorInsertOgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
UseCRISPRExpressionMammalianAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only