We narrowed to 62,913 results for: cloning

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AICSDP-15:ACTB-mEGFP

Plasmid

#87425

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

Depositor

Allen Institute for Cell Science

Publication

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

ACTB Homology Arms with mEGFP-linker (ACTB Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available since

March 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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Flag-HA-USP39

Plasmid

#22581

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP39 (USP39 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Flag-HA-UCHL3

Plasmid

#22564

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL3 (UCHL3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV-GfaABC1D-Cre-4x6T

Plasmid

#196410

Purpose

Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Has service

AAV5

Insert

Cre

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAd MafA-I-nGFP

Plasmid

#19412

Depositor

Douglas Melton

Publication

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pcDNA 3.1(-) mouse C/EBP delta

Plasmid

#12559

Depositor

Peter Johnson

Publication

McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

Insert

CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)

Expression

Mammalian

Mutation

S2G introduced during cloning

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pDonor_sU6

Plasmid

#69351

Purpose

sgRNA scaffold and synthetic U6 promoter

Depositor

Andrea Ventura

Publication

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

sU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

synthetic U6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

Plasmid

#154987

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-S5E2-dTom-nlsdTom

Plasmid

#135630

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has service

AAV1, AAV9, and AAV PHP.eB

Insert

dTom-nlsdTom

Use

AAV

Mutation

None

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hDlx-GiDREADD-dTomato-Fishell-5

Plasmid

#83896

Purpose

Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV1, AAV9, and AAV Retrograde

Insert

hM4Di

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCfuNca-MCS

Plasmid

#190820

Purpose

Multiple cloning site between Nitzschia captiva promoter and termination regions

Depositor

Peter Kroth

Publication

Sprecher et al Algal Research. 2023 May; Vol 72: 103136

Inserts

Multiple cloning site with SpeI and PstI
Bleomycin resistance gene

Expression

Bacterial

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1h

Plasmid

#113050

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

Depositor

Yulong Li

Publication

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has service

AAV9 and AAV Retrograde

Insert

GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)

Use

AAV

Promoter

hSyn

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pForge-KI-MMEJ-Cas9-DualGuide

Plasmid

#258792

Purpose

MMEJ Dual Nuclease

Depositor

Jay Moon Lee

Insert

hU6/7SK PITCh dual-guide cloning sites

Use

CRISPR

Promoter

hU6

Available since

Aug. 10, 2026

Availability

Academic Institutions and Nonprofits only

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OgeuIscB ωRNA-v2

Plasmid

#220958

Purpose

human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

Depositor

Weixin Tang

Publication

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning

Use

CRISPR

Expression

Mammalian

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1m

Plasmid

#113049

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

Depositor

Yulong Li

Publication

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has service

AAV9

Insert

GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)

Use

AAV

Promoter

hSyn

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-GRAB_NE1h

Plasmid

#123309

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

Depositor

Yulong Li

Publication

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Has service

AAV9 and AAV Retrograde

Insert

GPCR activation based NE sensor GRAB_NE1h

Use

AAV

Promoter

hSyn

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has service

AAV1 and AAV Retrograde

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pAAV-hsyn-GRAB_DA2h

Plasmid

#140554

Purpose

Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

Depositor

Yulong Li

Publication

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2h (high affinity)

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAV_hSyn1-SIO-stGtACR2-FusionRed

Plasmid

#105677

Purpose

Expresses a soma-targeted GtACR2 in Cre-expressing neurons

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has service

AAV1, AAV5, and AAV Retrograde

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

hSyn1

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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