We narrowed to 5,173 results for: pcDNA3.1+

1. pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

   Plasmid

   #18017

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu D154A Delta13V ECFP-W67G
   

   Use

   Tags

   ECFP-W67G and Venus

   Expression

   Mammalian

   Mutation

   mglB-D154A; non-functional ECFP

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

   Plasmid

   #13006

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 2 deltaCC (SULF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 88 to Alanine

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

3. XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

   Plasmid

   #16752

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-trunc before DEP (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncated before DEP

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP Rab11a S25N pcDNA3.1 Zeo (+)

   Plasmid

   #206080

   Purpose

   dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

   Depositor

   Annette Dolphin

   Article

   Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

   Insert

   RAB11A (RAB11A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S25N

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

   Plasmid

   #164011

   Purpose

   Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-mas-GRK2RH-Nluc L118A

   Plasmid

   #158606

   Purpose

   Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

   Plasmid

   #18016

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu D154A Delta13V Venus-Y67G
   

   Use

   Tags

   ECFP and Venus-Y67G

   Expression

   Mammalian

   Mutation

   mglB-D154A; non-functional Venus

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

   Plasmid

   #171429

   Purpose

   Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   FKBP-(GGS)X6-HaloTag C261
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

   Plasmid

   #171785

   Purpose

   Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-hH2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1V5-His Snk/Plk2 D223N

    Plasmid

    #22218

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, aspartic a…

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69899

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (stw Fly, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. (365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

    Plasmid

    #168188

    Purpose

    HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. NC4 pCDNA3.1 VENUS WTX (1-804)

    Plasmid

    #36959

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58

    Plasmid

    #171431

    Purpose

    Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    hH2A-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-KozATG-dH6.2-2XG4S-actin

    Plasmid

    #73263

    Purpose

    Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)

    Depositor

    Marcel Bruchez

    Article

    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

    Insert

    KozATG-dH6.2-2XG4S-actin (ACTB Synthetic, Human)
    

    Use

    Tags

    The FAP and actin are fused with 2 copies of a G4…

    Expression

    Mammalian

    Mutation

    The dH6.2 FAP has one cysteine in each domain cha…

    Promoter

    CMV

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MS2-HA-EGFP-HsNanos2_6-22_X

    Plasmid

    #147929

    Purpose

    Mammalian Expression of HsNanos2_6-22

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos2_6-22 (NANOS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-IL2sp-6His-Asprosin plasmid

    Plasmid

    #190683

    Purpose

    Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

    Depositor

    Atul Chopra

    Article

    Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

    Insert

    IL2-6xHis-Asprosin
    

    Use

    Tags

    IL2 signal peptide, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

    Plasmid

    #167313

    Purpose

    Template pDNA to prepare DD-CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    DD-CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-FLAG-Dicer-5'-pocket mutant

    Plasmid

    #51368

    Depositor

    Narry Kim

    Article

    Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    5' pocket mutant (R778A, R780A, R811A, H982A…

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

20. XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ

    Plasmid

    #16754

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3-trunc before PDZ (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncated before PDZ

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1 myc-mβTrCP F-box (1-247)

    Plasmid

    #62976

    Purpose

    Expresses myc-tagged mouse βTrCP residues 1-247

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    3x myc

    Expression

    Mammalian

    Mutation

    codes for residues 1-247

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

22. XE253 pCDNA3.1(zeo)-hDsh3-82-716

    Plasmid

    #16756

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-82-716-Dsh3 (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa 82-716

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1-FLAG-SBP-STAG2 (S97X)

    Plasmid

    #73964

    Purpose

    STAG2 cDNA (CCDS43990) S97X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S97X

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

24. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

    Plasmid

    #172581

    Purpose

    For transient expression of C-terminal BirA-tagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

    Plasmid

    #114112

    Purpose

    fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    NanoLuc; Volvox channelrhodopsin 1; EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

    Plasmid

    #127308

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FLAG-OTUB1 T134R,C91S-pcDNA3.1

    Plasmid

    #118212

    Purpose

    Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 91 to serine and threonine 134 t…

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

    Plasmid

    #64207

    Purpose

    mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 (CRY2 Mustard Weed)
    RGS4(del 1-33) (RGS4 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

30. Kv7.4 (1-645, Δ368-523)/pcDNA3.1

    Plasmid

    #111456

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523), C643A

    Promoter

    Available since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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