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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,358 results for: BLI;

Showing: 2441 - 2480 of 8358 results

  1. Clover2-C1

    Plasmid
    #54711
    Purpose
    Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Clover2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pAG416GAL-3x-uC-ccdB-6stop

    Plasmid
    #203514
    Purpose
    Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203514-plasmid-map-sequence-id-402866

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  3. pAG416GAL-2x-uA-ccdB-6stop

    Plasmid
    #203517
    Purpose
    Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203517-plasmid-map-sequence-id-402863

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  4. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

    Plasmid
    #137864
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137864-plasmid-map-sequence-id-274459

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  5. pKK1 -pFlp-18::mito4x-GCaMP6f

    Plasmid
    #194969
    Purpose
    Fluorescent reporter of mitochondrial matrix Ca2+.
    Depositor
    Frederic Hoerndli
    Insert
    4 repeats of Cox8 presequence plus GCaMP6f
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Flp-18
    Available since
    June 21, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194969-plasmid-map-sequence-id-388699

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  7. pET-(neg27)GFP-GGS9-Cre-6xHis

    Plasmid
    #89253
    Purpose
    Expresses GFP with -27 theoretical charge fused to Cre in E.Coli
    Depositor
    David Liu
    Insert
    (neg27)GFP-GGS9-Cre-6xHis
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89253-plasmid-map-sequence-id-176140

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  8. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

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  9. sCFP in pBSDONR P4r-P2

    Plasmid
    #121530
    Purpose
    For making C-terminal sCFP fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    sCFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    121530-plasmid-map-sequence-id-234035

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  10. pHAGE2-RSV-jGCaMP7s-W

    Plasmid
    #126690
    Purpose
    Mammalian calcium sensor protein.
    Depositor
    Darrell Kotton
    Insert
    jGCaMP7s
    Use
    Lentiviral
    Tags
    6xHIS, T7, Xpress
    Expression
    Mutation
    A52V, A317L
    Promoter
    RSV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126690-plasmid-map-sequence-id-245673

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  11. pUltra-Smurf-luc-RTTA3

    Plasmid
    #59438
    Purpose
    silence doxycyclin inducible pUltra-dox (also expresses luciferase)
    Depositor
    Yildirim Dogan, Kitai Kim
    Inserts
    luciferase
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    loxP5171
    Promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59438-plasmid-map-sequence-id-92494

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  12. pLV Tor1a-V5

    Plasmid
    #175135
    Purpose
    Lentiviral expression of Tor1a-Myc
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175135-plasmid-map-sequence-id-352022

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  13. pHAGE2-RSV-jGCaMP7f-W

    Plasmid
    #126691
    Purpose
    Mammalian calcium sensor protein.
    Depositor
    Darrell Kotton
    Insert
    jGCaMP7f
    Use
    Lentiviral
    Tags
    6xHIS, T7, Xpress
    Expression
    Mutation
    H78K, A317L
    Promoter
    RSV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126691-plasmid-map-sequence-id-245678

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  14. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

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  15. pBluescript mouse ptc

    Plasmid
    #58699
    Purpose
    full length mouse ptc cDNA
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pJK266

    Plasmid
    #73326
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73326-plasmid-map-sequence-id-135034

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  17. pIC81

    Plasmid
    #63065
    Purpose
    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    PMFI, IMAGE clone 6148250 (PMF1 Human)
    Use
    Tags
    EYFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63065-plasmid-map-sequence-id-104380

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  18. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

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  19. PTEN-VC

    Plasmid
    #89332
    Purpose
    expresses hPTEN fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    PTEN1 (PTEN Human)
    Use
    Tags
    C-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    89332-plasmid-map-sequence-id-176937

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  20. B0030m_BC

    Plasmid
    #74394
    Purpose
    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]
    Depositor
    Douglas Densmore
    Insert
    RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    74394-plasmid-map-sequence-id-140417

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  21. pSBtet-Bla/GFP

    Plasmid
    #198221
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198221-plasmid-map-sequence-id-389395

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  22. H2B CFP

    Plasmid
    #198059
    Purpose
    mCerulean fused to human H2B protein
    Depositor
    Amro Hamdoun
    Insert
    H2B
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mCerulean
    Expression
    Mutation
    Promoter
    sp6
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198059-plasmid-map-sequence-id-389151

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  23. pJK409

    Plasmid
    #72423
    Purpose
    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    72423-plasmid-map-sequence-id-135791

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  24. pJK405

    Plasmid
    #72418
    Purpose
    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72418-plasmid-map-sequence-id-135782

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  25. pJK403

    Plasmid
    #72416
    Purpose
    Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72416-plasmid-map-sequence-id-135537

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  26. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

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  27. MAC045-C12

    Plasmid
    #58753
    Purpose
    bacterial expression of PDB 4NXJ
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4NXJ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. KBB610

    Plasmid
    #185111
    Purpose
    Pom152 full repeat domain in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 Repeats-6His
    Promoter
    Available since
    Aug. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    185111-plasmid-map-sequence-id-368105

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  29. MB121 (Nf2 BBA)

    Plasmid
    #12170
    Depositor
    Tyler Jacks
    Insert
    Nf2 BBA (Nf2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    BBA: dominant negative Nf2, 7 Blue Box residues c…
    Promoter
    Available since
    July 7, 2006
    Availability
    Academic Institutions and Nonprofits only

  30. 371-Bc

    Plasmid
    #97028
    Purpose
    yORF-tev-His6
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97028-plasmid-map-sequence-id-182499

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  31. 371-B

    Plasmid
    #97027
    Purpose
    His6-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97027-plasmid-map-sequence-id-182504

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  32. MIGR1-PREP1

    Plasmid
    #78147
    Purpose
    retroviral expression of PREP1 and GFP
    Depositor
    Francesco Blasi
    Insert
    PREP1 (PKNOX1 Human)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. pHH0103 SMURF2 WW domain #3

    Plasmid
    #104226
    Purpose
    Bacterial expression of WW domain #3 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #3 (SMURF2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104226-plasmid-map-sequence-id-204295

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  34. pHH0103 SMURF2 WW domain #2

    Plasmid
    #104225
    Purpose
    Bacterial expression of WW domain #2 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #2 (SMURF2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104225-plasmid-map-sequence-id-204192

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  35. pHH0103 SMURF2 WW domain #1

    Plasmid
    #104224
    Purpose
    Bacterial expression of WW domain #1 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #1 (SMURF2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104224-plasmid-map-sequence-id-201513

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  36. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid
    #137859
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137859-plasmid-map-sequence-id-274442

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  37. pE-SUMO CRYGB

    Plasmid
    #80751
    Purpose
    Expression of human gamma B-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGB (CRYGB Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80751-plasmid-map-sequence-id-153519

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  38. mGeos2-VEL-Lifeact-7

    Plasmid
    #54817
    Purpose
    Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mGeos2-VEL-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pCB94

    Plasmid
    #69743
    Purpose
    Retroviral expression of PP2A beta
    Depositor
    Iain Cheeseman
    Insert
    PP2A beta (PTPA Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. pJK365

    Plasmid
    #72404
    Purpose
    Produces Acetobacter aceti 1023 Est01
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioester hydrolase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72404-plasmid-map-sequence-id-134631

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Showing: 2441 - 2480 of 8358 results