We narrowed to 2,903 results for: BFP

1. pAAV-CaMKII-mTagBFP2-P2A-DAAO-NES

   Plasmid

   #217566

   Purpose

   Expression of DAAO targeted to the cytoplasm and mTagBFP2, separated by P2A self-cleaving peptide, in mammalian neurons

   Depositor

   Vsevolod Belousov

   Article

   Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

   Insert

   TagBFP2-P2A-DAAO-NES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Oct. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2

   Plasmid

   #244171

   Purpose

   Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   IL10Ra signal peptide - 3xFLAG

   Expression

   Mammalian

   Mutation

   CTEVp_190K

   Promoter

   hEF1a

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2

   Plasmid

   #244174

   Purpose

   Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   MESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   VEGFR1 signal peptide - 3xFLAG

   Expression

   Mammalian

   Mutation

   CTEVp_190K

   Promoter

   hEF1a

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

   Plasmid

   #244178

   Purpose

   Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   TNFR1 signal peptide - 3xFLAG

   Expression

   Mammalian

   Mutation

   CTEVp_190K

   Promoter

   hEF1a

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

   Plasmid

   #244177

   Purpose

   Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   TNFR2 signal peptide - 3xFLAG

   Expression

   Mammalian

   Mutation

   CTEVp_190K

   Promoter

   hEF1a

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

   Plasmid

   #200539

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

   Plasmid

   #200540

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

   Plasmid

   #200541

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-ME_2a-BFP-pA ORF-1 without ATG

   Plasmid

   #200534

   Purpose

   mini circle middle entry plasmid for 2a-BFP-pA ORF 1 (without ATG) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-ME_2a-BFP-pA ORF-2 without ATG

    Plasmid

    #200535

    Purpose

    mini circle middle entry plasmid for 2a-BFP-pA ORF 2 (without ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC-ME_2a-BFP-pA ORF-3 without ATG

    Plasmid

    #200536

    Purpose

    mini circle middle entry plasmid for 2a-BFP-pA ORF 3 (without ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTOMM20-Rhobin2-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239022

    Purpose

    Co-overexpression of TOMM20-Rhobin2 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    William DeGrado, Bo Huang

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    TOMM20-Rhobin2 (TOMM20 Synthetic, Human)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTOMM20-Rhobin9-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239023

    Purpose

    Co-overexpression of TOMM20-Rhobin9 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    William DeGrado, Bo Huang

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    TOMM20-Rhobin9 (TOMM20 Synthetic, Human)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRhobin9-SEC61B-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239025

    Purpose

    Co-overexpression of Rhobin9-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    William DeGrado, Bo Huang

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    Rhobin9-SEC61B (SEC61B Synthetic, Human)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid

    #223106

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid

    #223107

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid

    #223108

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid

    #223109

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)

    Plasmid

    #233427

    Purpose

    PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    LIDARsensor(mcherry-BFP)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS3-rab-3P::AI::lite-1G::SL2::his-24::tagBFP

    Plasmid

    #232610

    Purpose

    Pan-neuronal expression of 'LITE-1' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    LITE-1 (lite-1 Nematode)
    

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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