We narrowed to 5,960 results for: plasmid dna
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Plasmid#140980PurposeEncodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasS-RLuc8
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsL-260-NLuc
Plasmid#207158PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsS-246-Nluc
Plasmid#207157PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD Mut
Plasmid#72218PurposeExpress flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
TagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain (cata…PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPb LAP
Plasmid#66979PurposeExpression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of proteinDepositorAvailable SinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RasAR(R89L)-NES
Plasmid#205568PurposeRasAR negative-control mutant plasmid. Targeted to cytosol.DepositorInsertRasAR(R89L)-NES (RAF1 Human, Synthetic)
TagsNuclear export signal (NES)ExpressionMammalianMutationArg 89 mutated to Leu in Raf1 RBD region.PromoterCMVAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HLA-cMyc-EcopT1R1
Plasmid#113962Purposemammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R1 (TAS1R1 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationtruncate N-terminal 24 residuesAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet2 CD Mut
Plasmid#72220PurposeExpress flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 2 (Tet2 Mouse)
TagsflagExpressionMammalianMutationThe plasmid expresses TET2 catalytic domain (cata…PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HLA-cmyc-natT1R2
Plasmid#113946Purposemammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R2 (TAS1R2 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationresidues 22-839Available SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HLA-Flag-natT1R2
Plasmid#113947Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationresidues 22-839Available SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10
Plasmid#181859PurposePart of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.DepositorInsertcp-PKN-mRuby2-GFP(1-0)
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt
Plasmid#157692Purposemammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
TagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
RGS-6xHis-BLMP-1-pcDNA3.1-
Plasmid#52514Purposeexpresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertblmp-1 (blmp-1 Nematode)
Tags6xHisExpressionMammalianMutationN75S mutation compared GenBank reference sequence…PromoterCMVAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-DNAJC13(351t)-tetramer
Plasmid#246668PurposeMammalian expression of truncated, eGFP tagged DNAJC13 (PH-like domain only), with exogenous tetramerization systemDepositorInsertDNAJC13 (DNAJC13 Human)
TagsArtificial tetramerization domain (Plasmid #12466…ExpressionMammalianMutationDeletion of residues 352 to 2243PromoterCMVAvailable SinceJan. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-DNAJC13(351t)-dimer
Plasmid#246667PurposeMammalian expression of truncated, eGFP tagged DNAJC13 (PH-like domain only), with exogenous dimerization systemDepositorInsertDNAJC13 (DNAJC13 Human)
TagsArtificial dimerization domain (Plasmid #124661) …ExpressionMammalianMutationDeletion of residues 352 to 2243PromoterCMVAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 JDM37-GFPnb-mCh
Plasmid#235666PurposeExpresses designed activator of Hsp70 tagged with a GFP nano body and mCherryDepositorInsertJDM37-GFPnb-mCh (PMCH Human, Synthetic)
TagsGFP nanobody and mCherryExpressionMammalianPromoterCMVAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO (pEHA1339)
Plasmid#209088PurposeBackbone plasmid for Tet Responsive transgene expression, FRT integrationDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF7(5’UTR)-FF
Plasmid#85490PurposeFirefly luciferase under the control of IRF7 5'UTRDepositorAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NDUFS6(5’UTR)-FF
Plasmid#85488PurposeFirefly luciferase under the control of NDUFS6 5'UTRDepositorAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-6His-mRplp1
Plasmid#173153Purposemouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cellsDepositorInsertORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) (Rplp1 Mouse)
TagsHisExpressionMammalianMutationWild typeAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only