We narrowed to 7,351 results for: ski

1. pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1

   Plasmid

   #202010

   Purpose

   Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   NbGP41P-sfGFP-VP64-GB1
   

   Use

   Synthetic Biology; Moclo level 1 vector

   Tags

   GB1 and sfGFP

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 GDI2

   Plasmid

   #67093

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GDI2 (GDI2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pVG122

   Plasmid

   #211765

   Purpose

   Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   strongLOV
   

   Use

   For single-copy integration (if cut with pmei)

   Tags

   SV40 NLS and VP16

   Expression

   Yeast

   Mutation

   compared to EL222, strongLOV contains Glu84Asp mu…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

   Plasmid

   #183804

   Purpose

   AAV vector to express nuclear localized eGFP from a Gad65 promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

   Insert

   eGFP-NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBetaActin-Halo-CAMSAP3

   Plasmid

   #191329

   Purpose

   Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   Halo-CAMSAP3 (Camsap3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

   Plasmid

   #182824

   Purpose

   Cre-dependent expression of cytosolic APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   APEX.NES-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EPB104

   Plasmid

   #68369

   Purpose

   contains human U6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   U6 promoter (RNU6-1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   U6

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. RhoC FLARE.sc mCer, mVenus - CA

   Plasmid

   #65424

   Purpose

   RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, Q63L in RhoC

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. RhoC FLARE.sc mCer, mVenus - DN

   Plasmid

   #65423

   Purpose

   RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, T19N in RhoC

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

10. NLS-HA-tdMCP-tdTomato

    Plasmid

    #183938

    Purpose

    tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdMCP-tdTomato (cp Synthetic)
    

    Use

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Vh-HA-3xFKBP

    Plasmid

    #20222

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFKBP (FKBP1A Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pAPM-D4 miR30-MORC2 ts1

    Plasmid

    #115886

    Purpose

    MORC2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    MORC2 shRNA (MORC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pscALPS-SIV-CPZ-TAN3 vpr

    Plasmid

    #115833

    Purpose

    Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVCPZ-TAN3 Vpr (DQ374658)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LNCX BMP-RII

    Plasmid

    #12641

    Depositor

    Rik Derynck

    Article

    Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

    Insert

    BMP-RII (BMPR2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLEX-FKBP-Rab5-Blasticidin

    Plasmid

    #120715

    Purpose

    Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Rab5a
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-215

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX-FKBP-Rab11-Blasticidin

    Plasmid

    #120717

    Purpose

    Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Rab11a
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-216

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMX-Flag-WIP

    Plasmid

    #11772

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pscALPS-SIV-CPZ-LB7 vpr

    Plasmid

    #115834

    Purpose

    Encodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVCPZ-LB7 Vpr (DQ373064)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pscALPS-SIV-GOR -vpr

    Plasmid

    #115835

    Purpose

    Encodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVGOR Vpr (FJ424871)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p944-SWITCH-OVER insert: EF1a-Puro

    Plasmid

    #217891

    Purpose

    insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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