We narrowed to 161,982 results for: addgene

1. KLHL3 (KLHL3A-c003)

   Plasmid

   #110251

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KLHL3 (KLHL3 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. XLone-BSD SARS-CoV2 N P2A mCherry

   Plasmid

   #154398

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SARS-CoV2 N Protein (N SARS-CoV2)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe puro IkB alpha M

   Plasmid

   #12332

   Depositor

   Inder Verma

   Insert

   IkB alpha M (Nfkbia Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Dominant-negative mutant. Many signal transductio…

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

4. 5VC9 (CXXC4)

   Plasmid

   #101263

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CXXC4 (CXXC4 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. KEAP1 (KEAP1A-c029)

   Plasmid

   #110247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KEAP1 (KEAP1 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-Flag-Mm Ezh2 Y641F

   Plasmid

   #80077

   Purpose

   Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

   Depositor

   Phillip Abbosh

   Inserts

   Flag-EZH2 Y641F (Ezh2 Mouse)
   Hygromycin phosphotransferase
   

   Use

   Retroviral

   Tags

   5' FLAG

   Expression

   Mammalian

   Mutation

   Y641F, R342R, N393N

   Promoter

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG306-GPD-ccdB chr I

   Plasmid

   #41894

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6BYB (L3MBTL1)

    Plasmid

    #119957

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pCLXSN-GFP

    Plasmid

    #27557

    Depositor

    Shao-Cong Sun

    Article

    Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 5TTW (EED)

    Plasmid

    #101254

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EED (EED Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. 5WCI (MYST1)

    Plasmid

    #101269

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MYST1 (KAT8 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAS_sfGFP150K

    Plasmid

    #193313

    Purpose

    pAS control plasmid for sfGFP expression

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    super folder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150K in sfGFP

    Promoter

    EF1

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEZYshuttle

    Plasmid

    #18703

    Purpose

    pShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB_EF1a_HA-VSVG

    Plasmid

    #212647

    Purpose

    piggyBac vector to express HA-VSVG

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    VSVG
    

    Use

    Piggybac

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ANO10 (TMEM16KA-c001)

    Plasmid

    #110267

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TMEM16K (ANO10 Human)
    

    Use

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid

    #41878

    Depositor

    Anna Huttenlocher

    Insert

    photoactivatable Rac1 (RAC1 Human)
    

    Use

    Zebrafish expression

    Tags

    6x His and mCherry

    Expression

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. CBX7

    Plasmid

    #25241

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX7_04 (CBX7 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pATF4.1.0-gDNA

    Plasmid

    #113772

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4

    Depositor

    Kevin White

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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