We narrowed to 13,583 results for: TIM

1. pNB649

   Plasmid

   #60136

   Purpose

   Firefly luciferase (Promega) driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-eIF4E-S200C

   Plasmid

   #37229

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   eIF4E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S200C

   Promoter

   T7

   Available since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pTYB2-eIF4B-A130C

   Plasmid

   #37227

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   TIF3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A130C

   Promoter

   T7

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pUC19-DAD4

   Plasmid

   #37224

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   DAD4 (DAD4 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pMIII-Neo

   Plasmid

   #153422

   Purpose

   MSCV with IRES-Neomycin resistant gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMV306G13+Lux

   Plasmid

   #26160

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   G13 promoter + Bacterial luciferase operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. Influenza-FPV-HA-mEGFP

   Plasmid

   #127810

   Purpose

   Expression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localization

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-link-yomKate2-SpHis5

   Plasmid

   #44852

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFPGUSPlus

   Plasmid

   #64401

   Purpose

   binary plant vector expressing GFP and GUS, both driven by a 35S promoter

   Depositor

   Claudia Vickers

   Article

   Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

   Inserts

   GFP
   GUSPlus
   

   Use

   Binary vector for plant transformation

   Tags

   His

   Expression

   Plant

   Mutation

   S65T

   Promoter

   35S

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-TPase

    Plasmid

    #51818

    Purpose

    For in vitro transcription of Tol2 transposase using T7 Promoter

    Depositor

    Elly Tanaka

    Article

    Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

    Insert

    Tol2 Transposase
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. psfaMs-Tn5

    Plasmid

    #183637

    Purpose

    Expresses anti-mouse nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti mouse secondary nanobody fused to Tn5
    

    Use

    Tags

    anti-mouse nanobody

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. psfaRb-Tn5

    Plasmid

    #183638

    Purpose

    Expresses anti-rabbit nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti rabbit secondary nanobody fused to Tn5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_ABEmax_P2A_GFP

    Plasmid

    #112101

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_BE4max_P2A_GFP

    Plasmid

    #112099

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SFG.wtCNa_opt.IRES.eGFP

    Plasmid

    #22489

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Sep 21. ():.

    Insert

    codon optimised wild type Calcineurin A (PPP3CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon Optimised wtCNa

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_AncBE4max_P2A_GFP

    Plasmid

    #112100

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p177bp-LacOx50-Hyg

    Plasmid

    #210213

    Purpose

    Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR58.6

    Plasmid

    #63176

    Purpose

    Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

    Plasmid

    #175540

    Purpose

    Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag9-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT/TO_HaloTag9_LamB1

    Plasmid

    #175538

    Purpose

    Expression of HaloTag9 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV_BE4max

    Plasmid

    #112093

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUNIV backbone

    Plasmid

    #24705

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Type

    Empty backbone

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV5-HA-mTmod3-WT

    Plasmid

    #70752

    Purpose

    N-terminal-HA-tagged mouse Tropomodulin3, WT

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Tropomodulin3 (Tmod3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV_ABEmax

    Plasmid

    #112095

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

    Plasmid

    #85225

    Purpose

    Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    John Naughton

    Inserts

    Optimized G Protein
    Enhanced GFP
    TVA (CD320 Chicken)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-oG

    Plasmid

    #74288

    Purpose

    To express oG (optimized Glycoprotein) under CAG promoter

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEB1-moxGFP

    Plasmid

    #103984

    Purpose

    Plasmid encoding moxGFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxGFP
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHis17_ParM _1

    Plasmid

    #78201

    Purpose

    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

    Insert

    parM (K33A I27C T174A T175N C287A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A I27C T174A T175N C287A

    Promoter

    unknown

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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