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Plasmid#141204PurposeChromobacterium violaceum transaminase with TEV cleaving site after the N-terminal Histag.DepositorInsertCvTA
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIT0.5m
Plasmid#128021PurposeR49A-K77ADepositorInsertCitA
UseTagsExpressionMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pTK234
Plasmid#13535DepositorInsertFLIPW-CTTY
UseTagsSee mapExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pAK005
Plasmid#233642PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagB
UseTags6-HisExpressionMammalianMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK004
Plasmid#233641PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagA
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pCYC1m_yeGFP
Plasmid#64389Purposeencodes yeast enhanced GFP under control of minimal pCYC1 promoterDepositorInsertyeast enhanced GFP
UseTagsExpressionYeastMutationPromoterpCYC1Available sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRPR1_gRNA_handle_RPR1t
Plasmid#49014PurposegRNA expression empty vector (yeast)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterpRPR1Available sinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet01
Plasmid#12172DepositorInsertNpuDnaE
UseTagsHis-GB1ExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#102951PurposeExpresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoterDepositorInsertAPEX2-NES
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tagged-Cap
Plasmid#35572DepositorTypeEmpty backboneUseLuciferaseTagsMs2 Stem LoopExpressionMammalianMutationPromoterSV40Available sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pAO139 (Apr LEU2 mrcl-AQ)
Plasmid#41924DepositorInsertMediator of the Replication Checkpoint
UseTagsExpressionMutationMRC1AQPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJH2971
Plasmid#100955PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAC200
Plasmid#187911Purposepsac200-crispey-bar-vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1664
Plasmid#112038PurposeCEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HISDepositorInsertSu9
UseTagsTagBFPExpressionYeastMutationamino acids 1-76PromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAO122 (Apr LEU2 MRC1)
Plasmid#41923DepositorInsertMediator of the Replication Checkpoint
UseTagsExpressionMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW1663
Plasmid#112037PurposeGal-Estradiol transcription activation expression cassette marked with KanRDepositorInsertGal DNA binding-estradiol binding-MSN2 activator
UseTagsExpressionYeastMutationPromoterADH1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
5LTR-puro-3LTR
Plasmid#86948PurposePiggyBac transposonDepositorInsertPiggyBac transposon
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
CIT32u
Plasmid#128019PurposeK92ADepositorInsertCitA
UseTagsExpressionMutationsee Depositor CommentsPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIT0
Plasmid#128017PurposeR66ADepositorInsertCitA
UseTagsExpressionMutationsee Depositor CommentsPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
1x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64390Purposeencodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(WT)
Plasmid#1146DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJW1505
Plasmid#62359PurposeRPL16a HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPL16A
UseYeast taggingTagsHA-TEV-AVIExpressionMutationPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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p405MET25
Plasmid#15961DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG416Gal-GFP
Plasmid#38309DepositorInsertEGFP (eGFP )
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
KaiC
Plasmid#41884DepositorInsertKai C
UseTagshistadineExpressionBacterialMutationPromotert7Available sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDBD-M
Plasmid#50572PurposeDouble Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571DepositorInsertDHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
UseTagsExpressionYeastMutationPromoterGal1Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRPR1(1xTetO)_gRNA_handle_RPR1t
Plasmid#62966PurposeaTc inducible gRNA expression vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterRPR1Available sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIPmal-40uDelta1YFP
Plasmid#18853DepositorInsertoptimized maltose FRET sensor
UseTagsCFP and YFPExpressionBacterialMutationN227A;PromoterAvailable sinceOct. 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
p316CUP1-3SGFP.SG
Plasmid#1207DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJW1660
Plasmid#112034PurposeC-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEUDepositorInsertIAA17
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterNoneAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426Gal-GFP
Plasmid#38310DepositorInsertEGFP (eGFP )
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH644-CENBEVY
Plasmid#29695DepositorInsertbidirectional promoter cassette from pBEVY-U
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pfbiRS
Plasmid#67283PurposeExpresses the fbiC gene from Mycobacterium smegmatisDepositorInsertfbiC gene from Mycobacterium smegmatis
UseTagspolyhistidineExpressionMammalianMutationnon knownPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L5-EGFP-L2
Plasmid#32593DepositorInsertEGFP (eGFP )
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
104b-U1(WT)
Plasmid#1153DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsGAL1:10 promoterExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pK218TK620T
Plasmid#1192DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationchanged lysine 218 to threonine and lysine 620 to…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJW1506
Plasmid#62360PurposeRPL16b HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPL16B
UseYeast targetingTagsHA-TEV-AVIExpressionMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMMDuet19
Plasmid#45594DepositorInsertNpuDnaB mini intein
UseTagsGB1 and H6-GB1ExpressionBacterialMutationremoval of endonuclease domainPromoterT7Available sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
6x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64392Purposeencodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only