We narrowed to 157,076 results for: Des

1. pDEST14-Avitag-SpyCatcher002

   Plasmid

   #102828

   Purpose

   Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Avitag-SpyCatcher002
   

   Tags

   Avitag, GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 373 pTol2-fli1ep:CherryDest

   Plasmid

   #73493

   Purpose

   Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 cassette, and late SV40 pA signal

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   fli1ep:mCherry-attR1-attR2
   

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-V5_Hu_p115-RhoGEF(WT)

   Plasmid

   #136335

   Purpose

   Mammalian expression of human p115-RhoGEF with N-terminal V5

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   ARHGEF1 (ARHGEF1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST17-hTRPT1-iso1

   Plasmid

   #184239

   Purpose

   For purification of N-terminally His-tagged TRPT1 from bacteria

   Depositor

   Karla Feijs

   Article

   Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

   Insert

   TRPT1 (TRPT1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 372 pTol2-fli1ep:EGFPDest

   Plasmid

   #73491

   Purpose

   Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   fli1ep:EGFP-attR1-attR2
   

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST40-SAR1-V5-His6

   Plasmid

   #67451

   Purpose

   Mammalian expression of hSar1 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SAR1 (SAR1A Human)
   

   Tags

   His and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLenti6/V5-DEST-RINT1

   Plasmid

   #40124

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   RINT1 (RINT1 Human)
   

   Use

   Lentiviral

   Tags

   V5 epitope

   Expression

   Mammalian

   Mutation

   L270M relative to NM_021930

   Promoter

   CMV

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6.3/TO/V5-DEST-IGFBP2

   Plasmid

   #191006

   Purpose

   Lentiviral expression vector for IGFBP2

   Depositor

   Johanna Ivaska

   Article

   Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

   Insert

   Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST47-ARL8A-GFP

   Plasmid

   #67403

   Purpose

   Mammalian expression of hArl8 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-Dest53-SHP-1 WT

    Plasmid

    #21791

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCFJ210 - pDESTR4-R3(ttTi4348, I)

    Plasmid

    #34863

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi4348 targeting
    

    Use

    Worm targeting

    Available Since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK069 desmaMCS:minprom_mApple,exorh:EGFP

    Plasmid

    #195982

    Purpose

    Expression construct

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC90-pmax-DEST

    Plasmid

    #48222

    Purpose

    Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR; Gateway destination

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST mfap4:tdTomato

    Plasmid

    #135214

    Purpose

    Red tagged macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter and tdTomato
    

    Use

    Zebrafish expression

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid

    #216286

    Purpose

    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ⍺-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST14-DoubleCatcher-β-Lock

    Plasmid

    #216287

    Purpose

    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher β-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST14-DoubleCatcher-γ-Lock

    Plasmid

    #216288

    Purpose

    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher γ-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST14-DoubleCatcher-δ-Lock

    Plasmid

    #216289

    Purpose

    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher δ-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST14-DoubleCatcher-ε-Lock

    Plasmid

    #216290

    Purpose

    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ε-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge