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AiP12098 - pAAV-AiE0374m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2098)

Plasmid

#208129

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12084 - pAAV-AiE0373m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2084)

Plasmid

#208128

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12495 - pAAV-AiE0385h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2495)

Plasmid

#208125

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12146 - pAAV-AiE0381h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2146)

Plasmid

#208124

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12144 - pAAV-AiE0376h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2144)

Plasmid

#208122

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12141 - pAAV-AiE0371h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2141)

Plasmid

#208121

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12979 - pAAV-AiE0361h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2979)

Plasmid

#208120

Purpose

AiE0361h is an enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S

Plasmid

#147478

Purpose

Mammalian Expression of HsUpf1-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1-S1107A-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

Plasmid

#178585

Purpose

Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

DLX2 (DLX2 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S

Plasmid

#147477

Purpose

Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 non silent mutations E167G, K282R, A442G and T1…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL52 (clone45)

Plasmid

#188578

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Use

Tags

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL52 (clone76)

Plasmid

#188579

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Use

Tags

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Enhancer-ZC3H12D_PromoterMUT

Plasmid

#153068

Purpose

ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutagenesis to disrupt 3 BHLHE40 binding sites. N…

Promoter

None

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Basic-ZC3H12D_PromoterMUT

Plasmid

#153067

Purpose

ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutagenesis to disrupt 3 BHLHE4040 binding sites.…

Promoter

None

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNT-15

Plasmid

#154932

Purpose

15 designed non-target sites

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

15 designed non-target sites

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNT-12

Plasmid

#154933

Purpose

12 designed non-target sites

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

12 designed non-target sites

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNT-3

Plasmid

#154934

Purpose

3 designed non-target sites

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

3 designed non-target sites

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2957

Plasmid

#222972

Purpose

To express a putative signal peptide from Encephalitozoon intestinalis protein Eint_060150 fused to GFP in mammalian cells

Depositor

Damian Ekiert, Gira Bhabha

Article

Jaroenlak et al bioRxiv 2024.05.30.596468

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2999

Plasmid

#222973

Purpose

To express a putative signal peptide from Encephalitozoon intestinalis protein Eint_060140 fused to GFP in mammalian cells

Depositor

Damian Ekiert, Gira Bhabha

Article

Jaroenlak et al bioRxiv 2024.05.30.596468

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL3000

Plasmid

#222974

Purpose

To express a putative signal peptide from Encephalitozoon intestinalis protein Eint_111330 fused to GFP in mammalian cells

Depositor

Damian Ekiert, Gira Bhabha

Article

Jaroenlak et al bioRxiv 2024.05.30.596468

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge