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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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9X NFAT-apha-MHC-Luc

Plasmid

#51941

Purpose

9 copies of NFAT sites in minimal alphaMHC-pGL-3

Depositor

Jeffery Molkentin

Article

Wilkins et al Circ Res. 2004 Jan 9;94(1):110-8. Epub 2

Inserts

9x NFAT binding site
alpha-MHC

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Hu

Plasmid

#61242

Purpose

Lentivirus expressing shRNA to human LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

LKB1 (STK11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-TER+-shLKB1-Hu

Plasmid

#61243

Purpose

Entry clone encoding shRNA to human LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

LKB1 (STK11 Human)

Use

Entr clone

Tags

Expression

Mutation

Promoter

H1/TO

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
PLKO.1-G3BP1-3'UTR

Plasmid

#136038

Purpose

G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p8103 LentiCRISPR v2 sgNT-2

Plasmid

#163313

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

sgNT-2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_C

Plasmid

#72622

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_834

Plasmid

#216087

Purpose

Cas9 [Sp] knockout targeting CD59, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD59 guide

Use

CRISPR and Lentiviral; Positive controls

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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PLKO.1-CAPRIN1-3'UTR

Plasmid

#136055

Purpose

CAPRIN1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCACGTTAGTGTCACAAATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA2

Plasmid

#134634

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA2 (UFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-STAG2 shRNA 1221

Plasmid

#31978

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

STAG2 (STAG2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
LEP-shMLL-AF9.1039

Plasmid

#105566

Purpose

retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

shRNA targeting MLL part of MLL-AF9 fusion

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_003 Intergenic control guide 2

Plasmid

#193585

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRNA control

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shSMAD4 #2

Plasmid

#83091

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
SL2-sgTelo-MTSa/EGFP/pdCas9-C1

Plasmid

#162759

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

Plasmid

#182347

Purpose

GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge