We narrowed to 34,848 results for: CaS;

1. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-RfxCas13d-HA

   Plasmid

   #141320

   Purpose

   Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCV-Cas9

   Plasmid

   #123643

   Purpose

   Expresses Cas9 with an N-terminal HUH-tag called PCV2

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Use

   Tags

   (EAAAK)8 linker and His6 + SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Promoter

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KRAB-dCas9

   Plasmid

   #112195

   Purpose

   KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   KRAB (ZNF10 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-KRAB-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-HF1

   Plasmid

   #138556

   Purpose

   Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEcCas-2.0

   Plasmid

   #190697

   Purpose

   Constitutive expressing of Cas9 and inducible expression of lambda Red

   Depositor

   Sheng Yang

   Article

   Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

   Insert

   Cas9 and Lamda Red system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9 TadCBEd

   Plasmid

   #193835

   Purpose

   Express TadCBEd (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Go1-CASE

   Plasmid

   #168123

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at L91/G92 within GNAO1

   Promoter

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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