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TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A

Plasmid

#212949

Purpose

Expresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Right-mouse-ATP6-1397C-AD

Plasmid

#212954

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-Kss1

Plasmid

#52682

Purpose

Expression of Kss1 in bacteria

Depositor

Benjamin Turk

Article

Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

Insert

KSS1 (KSS1 Budding Yeast)

Use

Tags

His6 and T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

Plasmid

#156452

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG

Depositor

Elphege Nora

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

RAD21, AID (Rad21 Mouse)

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

Plasmid

#175549

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

WAPL (Wapl Mouse)

Use

Mouse Targeting

Tags

AID[71-114]-eGFP

Expression

Mammalian

Mutation

none

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJK528

Plasmid

#71625

Purpose

Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)

Depositor

T. J. Kappock

Insert

phosphoribosylaminoimidazole carboxylase

Use

Tags

Expression

Bacterial

Mutation

changes methionine-105 to serine, isoleucine-109 …

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T2-Ste7-EE

Plasmid

#52681

Purpose

expression of constitutively active Ste7 kinase in bacteria

Depositor

Benjamin Turk

Article

Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

Insert

Ste7 (STE7 Budding Yeast)

Use

Tags

GST and Myc

Expression

Bacterial

Mutation

S359E/T363E constitutively active

Promoter

tac

Available since

April 30, 2014

Availability

Academic Institutions and Nonprofits only
CL20_mEGFP_ITSN2_ADCY3

Plasmid

#205832

Purpose

Express mEGFP-tagged fusion protein, ITSN2_ADCY3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ITSN2_ADCY3 (ITSN2, ADCY3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-MOR250-1

Plasmid

#22366

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR250-1 (Olfr109 Mouse)

Use

Tags

Rho

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pET20b-pIgR D1-H6

Plasmid

#12530

Depositor

Pamela Bjorkman

Article

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Use

Tags

His

Expression

Bacterial

Mutation

Lys 1-Val 109

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAcGP67A-pIgR D1-H6

Plasmid

#12531

Depositor

Pamela Bjorkman

Article

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Use

Tags

6xHis

Expression

Insect

Mutation

Lys 1 - Val 109

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pIVT-AIDm-EGFP

Plasmid

#182044

Purpose

in vitro transcription of AIDm-EGFP

Depositor

Janice Evans

Article

Camlin et al Biol Reprod. 2019 Oct 25;101(4):704-718.

Insert

AIDm-EGFP

Use

In vitro transcription

Tags

EGFP

Expression

Mutation

Promoter

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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HMEKc-srcM

Plasmid

#1107

Depositor

Susan Lindquist

Article

Xu et al Proc Natl Acad Sci U S A 1999 Jan 5;96(1

Insert

c-src

Use

Tags

Expression

Yeast

Mutation

MET3 promoter inserted in EcoRI and BamHI of pRS3…

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-FOXC1-MUT

Plasmid

#194567

Purpose

Mammalian Expression of mEGFP-FOXC1 Mutant (Q200Rfs*109) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXC1 (FOXC1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Q200Rfs*109

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKP112 (pRS406-GFP-ADH1t)

Plasmid

#106472

Purpose

Vector for creating C-terminal GFP fusion proteins in yeast.

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Type

Empty backbone

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

None

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAUC40

Plasmid

#71298

Purpose

Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

Depositor

Susanne von Bodman

Article

Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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Integrin-β6 Headpiece I270C

Plasmid

#159638

Purpose

Contains integrin beta 6 residues 1–474 with I270C mutation

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

Integrin beta 6 headpiece I270C

Use

Tags

6x His

Expression

Mammalian

Mutation

I270C

Promoter

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

Plasmid

#158793

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/109/03

Use

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Headpiece M400GC

Plasmid

#159637

Purpose

Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

alpha v headpiece M400GC

Use

Tags

6x His and Strep II

Expression

Mammalian

Mutation

M400C

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLz75_CB_operon_CsoS2_N3-N4

Plasmid

#140857

Purpose

Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 1-109 of CsoS2

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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