-
Plasmid#174068PurposeExpresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.DepositorInsertpTEF1-3NLS-97-3ZF-VP16
UseTagsFLAGExpressionYeastMutationPromoterpTEF1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1 WD
Plasmid#163580Purposeexpress Dcp2 ΔH1 WD at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationW50A, D54A, L255APromoterDCP2 promoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1
Plasmid#163576Purposeexpress Dcp2 ΔH1 (L255A) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationLysine at position 255 is mutated to AlaninePromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 FL-H1
Plasmid#163583Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationThe first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…PromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔN
Plasmid#163575Purposeexpress wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of N-terminal domain (1-242) of Dcp2PromoterDCP2 promoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300
Plasmid#163577Purposeexpress Dcp2 300 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domian (301-970)PromoterDCP2 promoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GXB
Plasmid#160926PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)DepositorInsertsBFP
sfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pJPS2954
Plasmid#135588PurposeSNR6 has UAUUUCat position 102–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ6 hasUAUUUCat position 102–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJPS2956
Plasmid#135590PurposeSNR6 has GAUUUAUUUC at position 98–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ10 has GAUUUAUUUC at position 98–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJPS2957
Plasmid#135591PurposeSNR6 has GAGAUUUAUUUC at position 96–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ12 has GAGAUUUAUUUC at position 96–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_U100C_U101C
Plasmid#81236PurposeU6 telestem mutant (U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with U100C_U101C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (G96C/A97C)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_A34G_A35G
Plasmid#81232PurposeU6 telestem mutant (A34G/A35G) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (A34G/A35G)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_G96C_A97C
Plasmid#81235PurposeU6 telestem mutant (G96C/A97C) for in vivo studiesDepositorInsertU6 RNA with G96C/A97C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (G96C/A97C)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP566
Plasmid#139500PurposeGene tagging vector to add mCherry Tag and membrane anchoring motifDepositorInsertmCherry-CaaX
UseTagsExpressionYeastMutationWTPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0317
Plasmid#128979PurposeEncodes Zif268DBD-CRY2 under pscADH1DepositorInsertpscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0320
Plasmid#128980PurposeEncodes Zif268DBD-CRY2PHR under pscADH1DepositorInsertpscADH1-SV40NLS-ZIF268DBD-CRY2PHR (L3)-tscADH1 TRP1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0521
Plasmid#128984PurposeExpression plasmid for ZIF268DBD-L3-CRY2DepositorInsertSV40NLS-Zif268DBD-CRY2 (L3) scTRP1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAEL003473 MIP R
Plasmid#115398PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertAAEL003473 MIP R (LOC5578188 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEG16*
Plasmid#111855PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL3p (promoter).DepositorInsertGAL3p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyTagsExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL3Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only