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pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

Plasmid

#84260

Purpose

Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgSV40
PYL1-KRAB

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Mutation

Promoter

CMV and mouse U6

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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AID-BFP-loxP_myo2_neoR

Plasmid

#194054

Purpose

Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Use

Tags

bfp

Expression

Worm

Mutation

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAGHa-SERBP1(189-400)

Plasmid

#172315

Purpose

Expression of the C-terminal (189-400) domain of SERBP1 in E. coli.

Depositor

David Libich

Article

Baudin et al Biomol NMR Assign. 2021 Oct;15(2):461-46

Insert

Plasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400 (SERBP1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted 1-188, 401-408

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:mVenus

Plasmid

#133970

Purpose

Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

mVenus

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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L1-1176

Plasmid

#13264

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Use

Tags

Expression

Bacterial

Mutation

Truncated before RSLE region

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-2T-FRB-W2027F

Plasmid

#73341

Purpose

Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)

Use

Tags

GST-tagged

Expression

Bacterial

Mutation

FRB domain only aa2015-2114; W2027F

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

Plasmid

#119316

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Only AA 481-852

Promoter

CMV

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T FRB Y2105A

Plasmid

#68726

Purpose

Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)

Use

Tags

GST-tagged

Expression

Bacterial

Mutation

FRB domain only aa2015-2114; Y2105A

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
BtARR2 Cys-less

Plasmid

#236273

Purpose

Bacterial expression of bovine arrestin-2 C59V, C125S, C140L, C150V, C242V, C251V, C269S

Depositor

Vsevolod Gurevich

Article

Zhuo et al J Biol Chem. 2014 Jul 25;289(30):20991-1

Insert

arrestin-2 (ARRB2 Bovine)

Use

Tags

Expression

Bacterial

Mutation

C59V, C125S, C140L, C150V, C242V, C251V, C269S

Promoter

lac

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Blast-PECAM1-Neo

Plasmid

#221149

Purpose

Expression of platelet and endothelial cell adhesion molecule 1 (CD31)

Depositor

Paul Burridge

Article

Pinheiro et al Sci Rep. 2024 Mar 26;14(1):7123. doi: 10

Insert

Platelet And Endothelial Cell Adhesion Molecule 1 (PECAM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PECAM1

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pML45

Plasmid

#206995

Purpose

Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

Ptbp1 gRNA (Ptbp1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBV-luc TOP2A 500bp promoter

Plasmid

#138273

Purpose

Luciferase reporter plasmid driven by TOP2A promoter

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A promoter sequence

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2001

Plasmid

#105871

Purpose

High copy HRPT backbone plasmid for insertion of MiniP promoters

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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gamma-tubulin (334-451)

Plasmid

#104436

Purpose

expressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin fragment 334 to 451 (TUBG1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

deleted a.a. 1 to 333

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA1_no stop

Plasmid

#83847

Purpose

Gateway donor vector for IAA1 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA1 (IAA1 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHL1954

Plasmid

#62831

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

M8::ryhB leader
hfq leader
gfp

Use

Synthetic Biology

Tags

m9::sodB leader and st7 RBS

Expression

Bacterial

Mutation

Promoter

pConNoHind, pLlacO-1, and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL1969

Plasmid

#62832

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

M8::ryhB leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::sodB leader

Expression

Bacterial

Mutation

Promoter

pConNoHind, pLlacO-1, and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2107

Plasmid

#62841

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

m8::micC leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::ompC leader

Expression

Bacterial

Mutation

Promoter

pConNoHind, pLlacO-1, and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only