Addgene

We narrowed to 16,714 results for: URE

Showing: 1261 - 1280 of 16714 results

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MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

Plasmid

#101190

Purpose

Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

F491A/M495A/T496A/N497A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

Plasmid

#101181

Purpose

Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/H698A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

Plasmid

#101182

Purpose

Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/Q695A/H698A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

Plasmid

#101183

Purpose

Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

M694A/Q695A/H698A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A

Plasmid

#101184

Purpose

Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mutation

G582A/V583A/E584A/D585A/N588A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

Plasmid

#101179

Purpose

Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/H698A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

Plasmid

#101180

Purpose

Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4D03

Plasmid

#70969

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf12 (Taf12 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK5 CBM-E

Plasmid

#53169

Purpose

Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

L280A/H281A/M323A/Q332A

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
pX330 p53

Plasmid

#59910

Purpose

pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting p53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pX330 Pten

Plasmid

#59909

Purpose

pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting Pten (Pten Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1

Plasmid

#97043

Purpose

Expresses RUNX1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1 (RUNX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
PB-tetO-NGN2

Plasmid

#197090

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2 (NEUROG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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MSP2443

Plasmid

#72252

Purpose

Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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plx307 SHOC2 V5 WT

Plasmid

#190568

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA10

Plasmid

#97042

Purpose

Expresses HOXA10 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A10 (HOXA10 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only
Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX330 Ctnnb1.1

Plasmid

#59911

Purpose

pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Plasmid

#101230

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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