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pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE
Plasmid#176269PurposeExpression of dCasMINI-V4-VPR-mCherry in mammalian cellsDepositorInsertdCasMINI-V4-VPR-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-EGFP-KASH
Plasmid#154373PurposeVector for Cre-dependent expression of EGFP tagged KASH protein domainDepositorInsertEGFP-KASH
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-2
Plasmid#129727PurposeExpressing SpCas9 and sgAAVS1-2DepositorInsertSpCas9 and sgAAVS1-2
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV298
Plasmid#204957PurposeAMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection markerDepositorInsertsMad7
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(D10A)
Plasmid#79618PurposeExpresses nCas9(D10A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSNG-P2A-Puro
Plasmid#136900PurposeLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)DepositorInsertFNLSNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationL1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337RPromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFiNG-P2A-Puro
Plasmid#136903PurposeLentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)DepositorInsertFNLSHiFiNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…PromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM098
Plasmid#216818PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmStayGold
UseTagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3ExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-EGFP-KASH
Plasmid#154374PurposeVector for Flp-dependent expression of EGFP tagged KASH protein domainDepositorInsertEGFP-KASH
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(H840A)
Plasmid#79616PurposeExpresses nCas9(H840A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationH840A for nickase Cas9PromoterpGal1Available sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L1-dMb2Cas12a
Plasmid#177683PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFi-P2A-Puro
Plasmid#136902PurposeLentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)DepositorInsertFNLSHiFi
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691APromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV302
Plasmid#204973PurposeAMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance markerDepositorInsertsMad7
nat1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV299
Plasmid#204967PurposeAMA1 plasmid with Aspergillus optimized Mad7 and argB selection markerDepositorInsertsMad7
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
SL3-MS2-gRNA GG backbone (MOBE3-4)
Plasmid#219949PurposeGolden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2DepositorInsertgRNA-MS2-SL3 (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'-com-gRNA GG backbone (MOBE1-4)
Plasmid#219947PurposeGolden gate backbone for mammalian expression of Sp-gRNA with 3'-comDepositorInsertgRNA-com-3'end (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only
