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Showing: 2561 - 2600 of 2924 results

  1. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hSyn
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-96396

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  2. AAV-KrasG12C/sgKras/Cre

    Plasmid
    #99850
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99850-plasmid-map-sequence-id-193772

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  3. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid
    #209060
    Purpose
    Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.
    Depositor
    Matthew Oser
    Article
    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.
    Insert
    sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
    Use
    Gateway vector to be used for lr reaction
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209060-plasmid-map-sequence-id-412264

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  4. AAV-KrasG12V/sgKras/Cre

    Plasmid
    #99854
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99854-plasmid-map-sequence-id-193790

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  5. AAV-KrasG13C/sgKras/Cre

    Plasmid
    #99856
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99856-plasmid-map-sequence-id-193794

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  6. AAV-KrasG12R/sgKras/Cre

    Plasmid
    #99852
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99852-plasmid-map-sequence-id-193774

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  7. AAV-KrasG13D/sgKras/Cre

    Plasmid
    #99857
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99857-plasmid-map-sequence-id-193798

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  8. AAV-KrasG12S/sgKras/Cre

    Plasmid
    #99853
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99853-plasmid-map-sequence-id-193755

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  9. AAV-KrasG12A/sgKras/Cre

    Plasmid
    #99849
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99849-plasmid-map-sequence-id-193789

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  10. AAV-KrasG13V/sgKras/Cre

    Plasmid
    #99860
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99860-plasmid-map-sequence-id-193770

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  11. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234448
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234448-plasmid-map-sequence-id-465743

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  12. AAV-KrasG13S/sgKras/Cre

    Plasmid
    #99859
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99859-plasmid-map-sequence-id-200267

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  13. AAV-KrasG13A/sgKras/Cre

    Plasmid
    #99855
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99855-plasmid-map-sequence-id-200262

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  14. AAV-KrasG13R/sgKras/Cre

    Plasmid
    #99858
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99858-plasmid-map-sequence-id-193748

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  16. pAAV-hDlx-Flex-GFP-Fishell_6

    Plasmid
    #83895
    Purpose
    Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV8, and AAV9
    Insert
    eGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hDlx
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    83895-plasmid-map-sequence-id-160320

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  19. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

    Plasmid
    #137960
    Purpose
    Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding yellow variant)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Y140A binding site mutation
    Promoter
    CAG
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137960-plasmid-map-sequence-id-271575

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  20. pAAV-hDlx-Flex-dTomato-Fishell_7

    Plasmid
    #83894
    Purpose
    Cre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hDlx
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    83894-plasmid-map-sequence-id-163144

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  21. pyrFEKO-HYG

    Plasmid
    #24020
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Floxed targeting vector for trypanosome
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24020-plasmid-map-sequence-id-11291

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  22. pyrFEKO-PUR

    Plasmid
    #24021
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Floxed targeting vector for trypanosome
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24021-plasmid-map-sequence-id-11292

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  23. pyrFEKO-BSD

    Plasmid
    #24024
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Floxed targeting vector for trypanosome
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24024-plasmid-map-sequence-id-11295

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  24. pyrFEKO-BLE

    Plasmid
    #24023
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Floxed targeting vector for trypanosome
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24023-plasmid-map-sequence-id-11294

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  25. pyrFEKO-NEO

    Plasmid
    #24022
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Floxed targeting vector for trypanosome
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24022-plasmid-map-sequence-id-11293

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  26. ZM (pyrFEKO-HYG TetR-T7RNAP)

    Plasmid
    #31455
    Depositor
    George Cross
    Insert

    Use
    Floxed targeting vector for trypanosome
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2011
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid
    #202199
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Has Service
    AAV1 and AAV5
    Insert
    stChrimsonR, PdCO
    Use
    AAV and Cre/Lox
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202199-plasmid-map-sequence-id-397078

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  29. MADR pDonor smFP-HA (dark) WPRE

    Plasmid
    #131007
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-HA WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    HA - 10 total HA epitope tages
    Expression
    Mammalian
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131007-plasmid-map-sequence-id-305185

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  30. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid
    #131006
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    V5 - 10 total of V5 epitope tag
    Expression
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131006-plasmid-map-sequence-id-305181

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  31. pMyt1GFPicre-Knock-In

    Plasmid
    #22657
    Depositor
    Lynn Hudson
    Insert
    Myelin Transcription Factor 1 (Myt1 Mouse)
    Use
    Targeting vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22657-plasmid-map-sequence-id-10369

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  32. pUltra-puro-RTTA3

    Plasmid
    #58750
    Purpose
    silence doxycyclin inducible pUltra-dox
    Depositor
    Kitai Kim, Yildirim Dogan
    Insert
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    loxP5171
    Promoter
    Ubiquitin C promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    58750-plasmid-map-sequence-id-92492

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  33. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid
    #100043
    Purpose
    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
    Depositor
    Ian Wickersham
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin-1
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100043-plasmid-map-sequence-id-193613

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  34. pAAV-EF1a-DIO-mCherry

    Plasmid
    #50462
    Purpose
    Double floxed mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    mCherry
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50462-plasmid-map-sequence-id-182309

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  35. pUltra-Smurf-luc-RTTA3

    Plasmid
    #59438
    Purpose
    silence doxycyclin inducible pUltra-dox (also expresses luciferase)
    Depositor
    Kitai Kim, Yildirim Dogan
    Inserts
    luciferase
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    loxP5171
    Promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59438-plasmid-map-sequence-id-92494

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  40. pAAV-hSyn-DIO-EGFP

    Plasmid
    #50457
    Purpose
    Double floxed EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV8
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50457-plasmid-map-sequence-id-162265

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Showing: 2561 - 2600 of 2924 results