Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,118 results for: Sis

Showing: 3201 - 3250 of 23118 results

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pCORE-Kp53

Plasmid

#72241

Purpose

Contains the CORE cassette with KanMX4 and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
kanMX4

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTX1-SMN1-exon7-ESE1

Plasmid

#126041

Purpose

In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN1 exon7 ESE1

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-mKdm4d(H189A)-polyA

Plasmid

#61554

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (catalytic mutant)

Tags

flag

Expression

Mammalian

Mutation

changed Histidine 189 (of Kdm4d) to Alanine

Promoter

CMV and T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Tags

8xHis

Expression

Bacterial

Promoter

PBAD30

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2xVlk1-3xFlag

Plasmid

#172773

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

Insert

Vlk1 (pkdcc.1.S Frog)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRecLV103-GFP-PGBD5

Plasmid

#65409

Purpose

Lentiviral expression vector for fusion of GFP with human PGBD5

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-UBC8

Plasmid

#104673

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC8 (UBC8 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCORE-Hp53

Plasmid

#72242

Purpose

Contains the CORE cassette with hyg and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
hyg

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pETCON-TNF

Plasmid

#67748

Purpose

Tumor Necrosis Factor Alpha Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

TNF-Alpha

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM2937

Plasmid

#84112

Purpose

target miRNA2937 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM2937

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM159

Plasmid

#84084

Purpose

target miRNA159 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM159

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941DR5-PacI-ath-STTM390

Plasmid

#84117

Purpose

target miRNA390 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM390

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM858a/b

Plasmid

#84177

Purpose

target miRNA858 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM858a/b

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM828

Plasmid

#84157

Purpose

target miRNA828 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM828

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only
pFGC5941-PacI-ath-STTM843

Plasmid

#84169

Purpose

target miRNA843 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM843

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM845

Plasmid

#84170

Purpose

target miRNA845 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM845

Expression

Plant

Promoter

2x35S

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM173

Plasmid

#84101

Purpose

target miRNA173 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM173

Expression

Plant

Promoter

2x35S

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
UG75-ADH1-DGK1-3HA

Plasmid

#198595

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UG75-ADH1-PAH1-3HA

Plasmid

#198597

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM168

Plasmid

#84093

Purpose

target miRNA168 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM168

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SH4-FRB-mRFP

Plasmid

#100741

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

Fyn

Tags

FRB and mRFP

Expression

Mammalian

Mutation

SH4 domain only

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM5026

Plasmid

#84192

Purpose

target miRNA5026 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM5026

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM398

Plasmid

#84124

Purpose

target miRNA398 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM398

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM395

Plasmid

#84121

Purpose

target miRNA395 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM395

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM773a/b

Plasmid

#84141

Purpose

target miRNA773a/b for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM773a/b

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_AtPHYB-S_X

Plasmid

#131183

Purpose

Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPHYB-S

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM408

Plasmid

#84132

Purpose

target miRNA408 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM408

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM161.1

Plasmid

#84086

Purpose

target miRNA161.1 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM161.1

Expression

Plant

Promoter

2x35S

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM396

Plasmid

#84122

Purpose

target miRNA396 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM396

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM779.2

Plasmid

#84145

Purpose

target miRNA779.2 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM779.2

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen

Plasmid

#174715

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutations

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

1-332, E151R, R154E

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM403

Plasmid

#84128

Purpose

target miRNA403 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM403

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM169a

Plasmid

#84094

Purpose

target miRNA169a for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM169a

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTX1-EV71-SL2

Plasmid

#126043

Purpose

In-vitro-transcription of Enterovirus 71 5UTR-SL2 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

Enterovirus 71 5'UTR SL2

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM829.2

Plasmid

#84158

Purpose

target miRNA829.2 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM829.2

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM162

Plasmid

#84088

Purpose

target miRNA162 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM162

Expression

Plant

Promoter

2x35S

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBJ

Plasmid

#62672

Purpose

Vector for FLPo mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FLPo

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM865-5p

Plasmid

#84186

Purpose

target miRNA865-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM865-5p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM399

Plasmid

#84125

Purpose

target miRNA399 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM399

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-SV40-puromycin

Plasmid

#138358

Purpose

Gateway compatible 3' entry clone contains puromycin driven by simian virus 40 (SV40)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

SV40-Puromycin

Expression

Mammalian

Promoter

The simian virus 40 (SV40) promoter

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM167

Plasmid

#84092

Purpose

target miRNA167 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM167

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM869.2

Plasmid

#84191

Purpose

target miRNA869.2 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM869.2

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM169d

Plasmid

#84095

Purpose

target miRNA169d for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM169d

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM863-3p

Plasmid

#84182

Purpose

target miRNA863-3p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM863-3p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM863-5p

Plasmid

#84183

Purpose

target miRNA863-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM863-5p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM161.2

Plasmid

#84087

Purpose

target miRNA161.2 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM161.2

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM775

Plasmid

#84142

Purpose

target miRNA775 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM775

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM827

Plasmid

#84156

Purpose

target miRNA827 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM827

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM831

Plasmid

#84161

Purpose

target miRNA831 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM831

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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